Cited 4 time in webofscience Cited 4 time in scopus

Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression

Title
Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression
Authors
Kar, GozdeKim, Jong KyoungKolodziejczyk, Aleksandra A.Natarajan, Kedar NathTriglia, Elena TorlaiMifsud, BorbalaElderkin, SarahMarioni, John C.Pombo, AnaTeichmann, Sarah A.
DGIST Authors
Kim, Jong Kyoung
Issue Date
2017-06-26
Citation
Nature Communications, 8
Type
Article
Article Type
Article
Keywords
AlleleAnimal Cell
ISSN
2041-1723
Abstract
Polycomb repressive complexes (PRCs) are important histone modifiers, which silence gene expression; yet, there exists a subset of PRC-bound genes actively transcribed by RNA polymerase II (RNAPII). It is likely that the role of Polycomb repressive complex is to dampen expression of these PRC-active genes. However, it is unclear how this flipping between chromatin states alters the kinetics of transcription. Here, we integrate histone modifications and RNAPII states derived from bulk ChIP-seq data with single-cell RNA-sequencing data. We find that Polycomb repressive complex-active genes have greater cell-to-cell variation in expression than active genes, and these results are validated by knockout experiments. We also show that PRC-active genes are clustered on chromosomes in both two and three dimensions, and interactions with active enhancers promote a stabilization of gene expression noise. These findings provide new insights into how chromatin regulation modulates stochastic gene expression and transcriptional bursting, with implications for regulation of pluripotency and development. © 2017 The Author(s).
URI
http://hdl.handle.net/20.500.11750/4143
DOI
10.1038/s41467-017-00052-2
Publisher
Nature Publishing Group
Related Researcher
Files:
There are no files associated with this item.
Collection:
New BiologyETC1. Journal Articles


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