Cited 1 time in webofscience Cited 1 time in scopus

Increased Excitatory Synaptic Transmission of Dentate Granule Neurons in Mice Lacking PSD-95-Interacting Adhesion Molecule Neph2/Kirrel3 during the Early Postnatal Period

Title
Increased Excitatory Synaptic Transmission of Dentate Granule Neurons in Mice Lacking PSD-95-Interacting Adhesion Molecule Neph2/Kirrel3 during the Early Postnatal Period
Authors
Roh, Junyeop D.Choi, Su-YeonCho, Yi SulChoi, Tae-YongPark, Jong-SilCutforth, TylerChung, WoosukPark, HanwoolLee, DongsooKim, Myeong-HeuiLee, YeunkumMo, SeojungRhee, Jeong-SeopKim, HyunKo, JaewonChoi, Se-YoungBae, Yong ChulShen, KangKim, EunjoonHan, Kihoon
DGIST Authors
Ko, Jaewon
Issue Date
2017-03-22
Citation
Frontiers in Molecular Neuroscience, 10
Type
Article
Article Type
Article
Keywords
Dentate Granule NeuronDentate Granule NeuronExcitatory SynapseExcitatory SynapseIntellectual DisabilityKirrel3Kirrel3LAR RPTPsMutationsNeph2NeuroliginsProtein CASKPSD 95Rat BrainReceptorSpecificitySynapses
ISSN
1662-5099
Abstract
Copy number variants and point mutations of NEPH2 (also called KIRREL3) gene encoding an immunoglobulin (Ig) superfamily adhesion molecule have been linked to autism spectrum disorders, intellectual disability and neurocognitive delay associated with Jacobsen syndrome, but the physiological roles of Neph2 in the mammalian brain remain largely unknown. Neph2 is highly expressed in the dentate granule (DG) neurons of the hippocampus and is localized in both dendrites and axons. It was recently shown that Neph2 is required for the formation of mossy fiber filopodia, the axon terminal structure of DG neurons forming synapses with GABAergic neurons of CA3. In contrast, however, it is unknown whether Neph2 also has any roles in the postsynaptic compartments of DG neurons. We here report that, through its C-terminal PDZ domain-binding motif, Neph2 directly interacts with postsynaptic density (PSD)-95, an abundant excitatory postsynaptic scaffolding protein. Moreover, Neph2 protein is detected in the brain PSD fraction and interacts with PSD-95 in synaptosomal lysates. Functionally, loss of Neph2 in mice leads to age-specific defects in the synaptic connectivity of DG neurons. Specifically, Neph2−/− mice show significantly increased spontaneous excitatory synaptic events in DG neurons at postnatal week 2 when the endogenous Neph2 protein expression peaks, but show normal excitatory synaptic transmission at postnatal week 3. The evoked excitatory synaptic transmission and synaptic plasticity of medial perforant pathway (MPP)-DG synapses are also normal in Neph2−/− mice at postnatal week 3, further confirming the age-specific synaptic defects. Together, our results provide some evidence for the postsynaptic function of Neph2 in DG neurons during the early postnatal period, which might be implicated in neurodevelopmental and cognitive disorders caused by NEPH2 mutations. © 2017 Roh, Choi, Cho, Choi, Park, Cutforth, Chung, Park, Lee, Kim, Lee, Mo, Rhee, Kim, Ko, Choi, Bae, Shen, Kim and Han.
URI
http://hdl.handle.net/20.500.11750/4211
DOI
10.3389/fnmol.2017.00081
Publisher
Frontiers Research Foundation
Related Researcher
  • Author Ko, Jae Won Laboratory of Synapse Formation and Function
  • Research Interests Neuroendocine Regulation by Gut-Microbiota derived Small Chain fatty Acids (SCFA); Neural Immune Regulation by Medium/Small Chain Fatty Acids (MCFA); Odorant G-protein Coupled Receptors (GPCRs) Structure and Function; Ectopic Odorant GPCRs and Endocrine Diseases
Files:
There are no files associated with this item.
Collection:
ETC1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE