Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Huang, Yun | - |
dc.contributor.author | Kim, Jong Kyoung | - |
dc.contributor.author | Dang Vinh Do | - |
dc.contributor.author | Lee, Caroline | - |
dc.contributor.author | Penfold, Christopher A. | - |
dc.contributor.author | Zylicz, Jan J. | - |
dc.contributor.author | Marioni, John C. | - |
dc.contributor.author | Hackett, Jamie A. | - |
dc.contributor.author | Surani, M. Azim | - |
dc.date.available | 2017-08-10T08:15:50Z | - |
dc.date.created | 2017-08-09 | - |
dc.date.issued | 2017-03 | - |
dc.identifier.issn | 2050-084X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/4214 | - |
dc.description.abstract | The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional misregulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression. © Huang et al. | - |
dc.publisher | eLife Sciences Publications Ltd | - |
dc.title | Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition | - |
dc.type | Article | - |
dc.identifier.doi | 10.7554/eLife.22345 | - |
dc.identifier.scopusid | 2-s2.0-85018265565 | - |
dc.identifier.bibliographicCitation | eLife, v.6 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordPlus | Differential Expression | - |
dc.subject.keywordPlus | DNA Methylation | - |
dc.subject.keywordPlus | Early Mouse Development | - |
dc.subject.keywordPlus | Gene Expression | - |
dc.subject.keywordPlus | Host Genes | - |
dc.subject.keywordPlus | Human Preimplantation Embryos | - |
dc.subject.keywordPlus | RNA Seq Experiments | - |
dc.subject.keywordPlus | Single Cell | - |
dc.subject.keywordPlus | Stem Cells | - |
dc.subject.keywordPlus | Transposable Elements | - |
dc.citation.title | eLife | - |
dc.citation.volume | 6 | - |