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Transcription factor NFAT5 promotes macrophage survival in rheumatoid arthritis

Title
Transcription factor NFAT5 promotes macrophage survival in rheumatoid arthritis
Authors
Choi, SusannaYou, SungyongKim, DonghyunChoi, Soo YounKwon, H. MooKim, Hyun-SookHwang, DaeheePark, Yune-JungCho, Chul-SooKim, Wan-Uk
DGIST Authors
Hwang, Daehee
Issue Date
2017-03-01
Citation
Journal of Clinical Investigation, 127(3), 954-969
Type
Article
Article Type
Article
Keywords
ActivationApoptosisClodronate Containing LiposomesDisease ActivityInflammatory ArthritisLeukemia CellsMonocyte Chemoattractant Protein 1Osmotic StressProliferationSynovial Tissue
ISSN
0021-9738
Abstract
Defective apoptotic death of activated macrophages has been implicated in the pathogenesis of rheumatoid arthritis (RA). However, the molecular signatures defining apoptotic resistance of RA macrophages are not fully understood. Here, global transcriptome profiling of RA macrophages revealed that the osmoprotective transcription factor nuclear factor of activated T cells 5 (NFAT5) critically regulates diverse pathologic processes in synovial macrophages including the cell cycle, apoptosis, and proliferation. Transcriptomic analysis of NFAT5-deficient macrophages revealed the molecular networks defining cell survival and proliferation. Proinflammatory M1-polarizing stimuli and hypoxic conditions were responsible for enhanced NFAT5 expression in RA macrophages. An in vitro functional study demonstrated that NFAT5-deficient macrophages were more susceptible to apoptotic death. Specifically, CCL2 secretion in an NFAT5-dependent fashion bestowed apoptotic resistance to RA macrophages in vitro. Injection of recombinant CCL2 into one of the affected joints of Nfat5+/-mice increased joint destruction and macrophage infiltration, demonstrating the essential role of the NFAT5/CCL2 axis in arthritis progression in vivo. Moreover, after intra-articular injection, NFAT5-deficient macrophages were more susceptible to apoptosis and less efficient at promoting joint destruction than were NFAT5-sufficient macrophages. Thus, NFAT5 regulates macrophage survival by inducing CCL2 secretion. Our results provide evidence that NFAT5 expression in macrophages enhances chronic arthritis by conferring apoptotic resistance to activated macrophages.
URI
http://hdl.handle.net/20.500.11750/4222
DOI
10.1172/JCI87880
Publisher
American Society for Clinical Investigation
Related Researcher
  • Author Hwang, Dae Hee Systems Biology and Medicine Lab
  • Research Interests Multilayered spatiotemporal networks; Regulatory motifs or pathways; Metabolite-protein networks; Network stochasticity; Proteomics and informatics
Files:
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Collection:
New BiologyETC1. Journal Articles
ETC1. Journal Articles


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