Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jae Kyoo | - |
dc.contributor.author | Nam, Hong Gil | - |
dc.contributor.author | Zare, Richard N. | - |
dc.date.available | 2017-08-10T08:17:36Z | - |
dc.date.created | 2017-08-09 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 0033-5835 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/4240 | - |
dc.description.abstract | Kinetics of acid-induced chlorophyll demetallation was recorded in microdroplets by fusing a stream of microdroplets containing 40 μM chlorophyll a or b dissolved in methanol with a stream of aqueous microdroplets containing 35 mM hydrochloric acid (pH = 1-46). The kinetics of the demetallation of chlorophyll in the fused microdroplets (14 ± 6 μm diameter; 84 ± 18 m s-1 velocity) was recorded by controlling the traveling distance of the fused microdroplets between the fusion region and the inlet of a mass spectrometer. The rate of acid-induced chlorophyll demetallation was about 960 ± 120 times faster in the charged microdroplets compared with that reported in bulk solution. If no voltage was applied to the sprayed microdroplets, then the acceleration factor was about 580 ± 90, suggesting that the applied voltage is not a major factor determining the acceleration. Chlorophyll a was more rapidly demetallated than chlorophyll b by a factor of ∼26 in bulk solution and ∼5 in charged microdroplets. The demetallation kinetics was second order in the H+ concentration, but the acceleration factor of microdroplets compared with bulk solution appeared to be unchanged in going from pH = 1-3 to 7-0. The water:methanol ratio of the fused microdroplets was varied from 7:3 to 3:7 causing an increase in the reaction rate of chlorophyll a demetallation by 20%. This observation demonstrates that the solvent composition, which has different evaporation rates, does not significantly affect the acceleration. We believe that a major portion of the acceleration can be attributed to confinement effects involving surface reactions rather than either to evaporation of solvents or to the introduction of charges to the microdroplets. © Cambridge University Press 2017. | - |
dc.language | English | - |
dc.publisher | Cambridge University Press | - |
dc.title | Microdroplet fusion mass spectrometry: Accelerated kinetics of acid-induced chlorophyll demetallation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1017/S0033583517000014 | - |
dc.identifier.scopusid | 2-s2.0-85034422336 | - |
dc.identifier.bibliographicCitation | Quarterly Reviews of Biophysics, v.50, pp.1 - 7 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordPlus | Charged Microdroplets | - |
dc.subject.keywordPlus | Electrospray | - |
dc.subject.keywordPlus | Neat Water | - |
dc.subject.keywordPlus | Water Surface | - |
dc.citation.endPage | 7 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Quarterly Reviews of Biophysics | - |
dc.citation.volume | 50 | - |
There are no files associated with this item.