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Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana

Title
Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana
Authors
Khokon, Md. A. R.Salam, M. A.Jammes, F.Ye, W.Hossain, M. A.Uraji, M.Nakamura, Y.Mori, I. C.Kwak, J. M.Murata, Y.
DGIST Authors
Kwak, J. M.
Issue Date
2015-09
Citation
Plant Biology, 17(5), 946-952
Type
Article
Article Type
Article
Keywords
[Ca2+](Cyt) OscillationsABAAbscisic Acid (ABA)Anion Channel Slac1Cytosolic AlkalisationCytosolic CalciumGuard CellIon ChannelMap KinaseMethyl JasmonateMitogen Activated Protein KinaseOxygen Species ProductionPhosphatase PairReactive Oxygen Species (ROS)RolesS Type Anion ChannelsSignal Transduction
ISSN
1435-8603
Abstract
Methyl jasmonate (MeJA) and abscisic acid (ABA) signalling cascades share several signalling components in guard cells. We previously showed that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signalling in Arabidopsis thaliana. In this study, we examined whether these two MAP kinases function in MeJA signalling using genetic mutants for MPK9 and MPK12 combined with a pharmacological approach. MeJA induced stomatal closure in mpk9-1 and mpk12-1 single mutants as well as wild-type plants, but not in mpk9-1 mpk12-1 double mutants. Consistently, the MAPKK inhibitor PD98059 inhibited the MeJA-induced stomatal closure in wild-type plants. MeJA elicited reactive oxygen species (ROS) production and cytosolic alkalisation in guard cells of the mpk9-1, mpk12-1 and mpk9-1 mpk12-1 mutants, as well in wild-type plants. Furthermore, MeJA triggered elevation of cytosolic Ca2+ concentration ([Ca2+](cyt)) in the mpk9-1 mpk12-1 double mutant as well as wild-type plants. Activation of S-type anion channels by MeJA was impaired in mpk9-1 mpk12-1. Together, these results indicate that MPK9 and MPK12 function upstream of S-type anion channel activation and downstream of ROS production, cytosolic alkalisation and [Ca2+](cyt) elevation in guard cell MeJA signalling, suggesting that MPK9 and MPK12 are key regulators mediating both ABA and MeJA signalling in guard cells.
URI
http://hdl.handle.net/20.500.11750/4296
DOI
10.1111/plb.12321
Publisher
WILEY-BLACKWELL
Related Researcher
  • Author Kwak, June Myoung KWAK_LAB(Lab of Cell Signailng)
  • Research Interests Calcium Signaling; ABA;ROS Signaling; drought-tolerant plants; Development of Abscission; Culluar Features; Signaling network; Environmental Regulation
Files:
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Collection:
New BiologyETC1. Journal Articles


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