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The role of NdgR in glycerol metabolism in Streptomyces coelicolor

Title
The role of NdgR in glycerol metabolism in Streptomyces coelicolor
Authors
Lee, Bo-RahmBhatia, Shashi KantSong, Hun-SukKim, JunyoungKim, WooseongPark, Hyung-yeonYoon, Jeong-JunPark, Sung-HeeHwang, DaeheeKim, Byung-GeeYang, Yung-Hun
DGIST Authors
Hwang, Daehee
Issue Date
2017
Citation
Bioprocess and Biosystems Engineering, 40(10), 1573-1580
Type
Article
Article Type
Article
Keywords
A3(2)AlcoholsAmino AcidsAntibiotic ProductionAntibioticsBacteriaFatty Acid ProductionsFatty AcidsGelRetardation AssayGenesGeneticsGlycerolMetabolismN AcetylglucosamineNdgROperonPharmaceutical AntibioticsPhysiologyProteinRalstonia EutrophaRegulatorRenewable FuelsRepressionStreptomyces CoelicolorStressSubstrate Induction
ISSN
1615-7591
Abstract
Streptomyces, which produces many pharmaceutical antibiotics and anticancer agents, is a genus of soil-dwelling bacteria with numerous regulators that control both primary and secondary metabolism. NdgR is highly conserved in Streptomyces spp. and is known to be involved in antibiotic production, tolerance against shock and physical stress, nitrogen metabolism, leucine metabolism, and N-acetylglucosamine metabolism. As another function of NdgR, we report the involvement of NdgR in glycerol metabolism in S. coelicolor. Initially, a glycerol utilization operon containing gylCABX was found to be up-regulated in an ndgR deletion mutant (BG11) grown in N-acetylglucosamine solid minimal media compared with wild-type strain (M145). BG11 produced more antibiotics with a small amount of glycerol and increased glycerol utilization, yielding higher concentrations of lactate and acetate per cell. Moreover, fatty acid production was also changed in BG11 to produce longer chain fatty acids, phenolic compounds, alkanes, and fatty alcohols. Using a gel retardation assay, NdgR was found to bind the upstream region of gylC, working as a repressor. NdgR is a second regulator of a glycerol utilization operon, for which only one regulator, GylR was previously known. © 2017, Springer-Verlag GmbH Germany.
URI
http://hdl.handle.net/20.500.11750/4423
DOI
10.1007/s00449-017-1813-z
Publisher
Springer Verlag
Files:
There are no files associated with this item.
Collection:
ETC1. Journal Articles


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