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Biofunctionalized graphene oxide wrapped carbon nanotubes enabled microfluidic immunochip for bacterial cells detection

Title
Biofunctionalized graphene oxide wrapped carbon nanotubes enabled microfluidic immunochip for bacterial cells detection
Authors
Singh, ChandanAli, AzaharReddy, VenuSingh, DineshKim, Cheol-GiSumana, G.Malhotra, B.D.
DGIST Authors
Kim, Cheol-Gi
Issue Date
2018-02
Citation
Sensors and Actuators, B: Chemical, 255, 2495-2503
Type
Article
Article Type
Article
Keywords
AntibodiesCarbonCarbon NanotubesCarboxylated Multi-Walled Carbon NanotubesElectron Transfer BehaviorEscherichia ColiGrapheneGraphene OxideGraphene OxidesIndium Tin Oxide ElectrodesMicrofluidic ImmunochipMicrofluidicsMultiwalled Carbon Nanotubes (MWCN)NanosheetsNanotubesPolydimethylsiloxanePolydimethylsiloxane PDMSSalmonellaSalmonella TyphimuriumSensitivitySiliconesSynergistic EffectTin OxidesYarn
ISSN
0925-4005
Abstract
A sensitive and selective microfluidic immunochip was fabricated for detection of Salmonella typhimurium (S. typhimurium) bacterial cells. In this sensor, graphene oxide (GO) nano sheets wrapped carboxylated multiwalled carbon nanotubes (cMWCNTs) composite acted as a transducer material. The colloidal solution of GO-cMWCNTs composite was selectively deposited onto patterned indium tin oxide (ITO) electrode and sealed with polydimethylsiloxane (PDMS) micro channels. The S. typhimurium antibodies (StAb) were in situ biofunctionalized followed by EDC-NHS covalent chemistry via amidation reaction. The presence of abundant functional groups at the GO-cMWCNTs composite improved the loading of antibodies (StAb) against S. typhimurium leading to improved biosensing characteristics. Wrapping of cMWCNTs with GO resulted in superior electron transfer behavior enhancing the sensitivity (162.47μA/CFU-1/mLcm-2) almost two folds as compared to that based on GO (89.16μA/CFU-1/mLcm-2) sheets for bacterial cells detection. Besides this, GO wrapped cMWCNTs integrated microfluidics biosensor offered low detection limit as 0.376 CFU/mL and negligible interference due to presence of Escherichia coli (E. coli (O157:H7). © 2017 Elsevier B.V.
URI
http://hdl.handle.net/20.500.11750/4563
DOI
10.1016/j.snb.2017.09.054
Publisher
Elsevier B.V.
Files:
There are no files associated with this item.
Collection:
Emerging Materials ScienceLab for NanoBio-Materials & SpinTronics(nBEST)1. Journal Articles


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