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Effects of cross-sectional change on the isotachphoresis process for protein-separation chip design
- Effects of cross-sectional change on the isotachphoresis process for protein-separation chip design
- Choi, Hongsoo; Jeon, Youngwon; Cho, Migyung; Lee, Dong-yeon; Shim, Jaesool
- DGIST Authors
- Choi, Hongsoo
- Issue Date
- Microsystem Technologies, 16(11), 1931-1938
- Article Type
- RESOLUTION COMPUTER-SIMULATION; ELECTROPHORETIC MOBILIZATION; ISOTACHOPHORESIS; MICROCHIP
- A 2D finite volume method (FVM)-based computer simulation model has been developed for isota-chophoresis (ITP) in three different 20 mm long micro-channels to assist the design of a protein separation chip. The model is based on three major equations, i.e. the mass conservation, charge conservation and electro-neutrality equations. In this study, the ITP system has four negatively-charged components, namely, hydrochloric acid, caproic acid, acetic acid, and benzoic acid, and one positively-charged component, namely, histidine, for use as a background electrolyte (BE). The calculations were performed under the action of a nominal electric field of -5,000 V/m. For the validation of our model, the results of our simulation in a straight channel are compared with the results of a 1D-based open program (SIMUL5), and all the physico-chemical properties are obtained from the SIMUL5. Unlike 1D ITP separation, spatially-changed micro-channel shapes provided different separation and moving times as well as a quasi steady state time compared to the 1D results obtained during the ITP process. Dispersion analysis is also conducted using a 2D moment analysis to investigate the effect of 2D geometries on ITP separation. © Springer-Verlag 2010.
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