Skeletal muscle function plays a very important role in quality of life. However, skeletal muscle causes functional decline under aging or some diseases. Exercise and muscle training are good solutions to delay sarcopenia, but there are limitations to those who are uncomfortable in exercise. For this reason, alternative interventions for muscle sarcopenia are required, and many studies proved the increase of skeletal muscle mass by electrical stimulation. In conventional studies, however, mouse skeletal muscle cells have been mostly used in experiments to identify electrical stimulation conditions while human derived cells have not been frequently utilized in these studies. Stimulation used for rehabilitation has been uniformly treated without the consideration of aging. In addition, many studies have been used with conventional petri dish usually requiring many numbers of cells, which is not appropriate for rare. Moreover, they are not usually condition uniformity of electrical field. In this study, we have developed an electrical stimulation device which consumes a small amount of cells and can form a uniform electrical field. With the system, we analyzed the skeletal muscle differentiation and Myotube thickness depending on the electrical stimulation condition.