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Rapid detection of 6x-histidine-labeled recombinant proteins by immunochromatography using dye-labeled cellulose nanobeads

Title
Rapid detection of 6x-histidine-labeled recombinant proteins by immunochromatography using dye-labeled cellulose nanobeads
Authors
Choi, Eun-SookLee, Se GeunLee, Sung-JunKim, Eunjoo
DGIST Authors
Lee, Se GeunLee, Sung-JunKim, Eunjoo
Issue Date
2015-03
Citation
Biotechnology Letters, 37(3), 627-632
Type
Article
Article Type
Article
Keywords
Amino AcidsAntibodiesCelluloseChemical AnalysisChemistry Techniques, AnalyticalColorimetryDye-Labeled Cellulose NanobeadsEscherichia ColiHexa-Histidine TagHis-Tagged ProteinsHybrid ProteinImmunoaffinity ChromatographyImmunochromatographyImmunochromatography AssaysInverse RelationshipMetabolismNano-Structured MaterialsNanobeadsNanoparticleNanoparticlesProceduresRapid TestRecombinant Fusion ProteinsRecombinant Protein DetectionRecombinant ProteinsTimeTime FactorsWestern Blotting
ISSN
0141-5492
Abstract
A rapid and easy immunochromatography assay using dye-labeled cellulose nanobeads (CNBs) was developed to detect proteins with hexa-histidine tag (His-tag) to characterize recombinant proteins during purification. Recombinant ATG8 protein was used as a His-tagged protein, and ATG8-conjugated CNBs (A-CNBs) were prepared. The original ATG8 in the sample solution competed with A-CNBs for anti-His-tag antibodies spotted on to the strip resulting in an inverse relationship between ATG8 concentration and the colorimetric signal. The usefulness of this method was shown by adding ATG8 to a 1 % Escherichia coli extract. In addition, this assay can be used to detect other His-tagged proteins without protein-specific antibodies. Because the identification of fractions containing His-tagged proteins by western blotting or ELISA is labor-intensive and expensive, our method provides an efficient and cheaper alternative. © 2014, Springer Science+Business Media Dordrecht.
URI
http://hdl.handle.net/20.500.11750/5205
DOI
10.1007/s10529-014-1731-y
Publisher
Springer
Related Researcher
Files:
There are no files associated with this item.
Collection:
Magnet-Controlled Materials Research Group1. Journal Articles
Companion Diagnostics and Medical Technology Research Group1. Journal Articles


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