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Bacteriophages as Templates for Manufacturing Supramolecular Structures
- Bacteriophages as Templates for Manufacturing Supramolecular Structures
- Korkmaz, Nuriye; Kim, Young Jun; Nam, Chang Hoon
- DGIST Authors
- Nam, Chang Hoon
- Issue Date
- Macromolecular Bioscience, 13(3), 376-387
- Article Type
- Amino Acids; Animal Cell; Apatite; Apatite-Like Materials; Atomic Force Microscopy; Bacteriophage; Bacteriophages; Bio-Compatible Materials; Bioengineering; Biomineralization; Calcium; Calcium Chloride; Capsid Proteins; Chemical Reduction; Coat Proteins; Cobalt; Cobalt Chloride; Controlled Study; EDX Spectroscopy; Electron Microscopy; Energy Dispersive X Ray Spectroscopy; Escherichia Coli; Immunofluorescence Analysis; Immunofluorescence Microscopy; Macromolecular Substances; Metallation; Metallization; Metallizing; Microscopy, Atomic Force; Microscopy, Electron, Scanning; Molecular Scaffold; N-Terminals; Nanofabrication; Negative Surface Charges; Non-Human; Phage Bundles; Phage Display; Phosphorus; Recombinant Proteins; Reduction; Scanning Electron Microscopy; Static Electricity; Supramolecular Chemistry; Supramolecular Structure; Surface Properties; Virus Assembly; X Ray Spectrometry; Zeta Potential; Zeta Potential Measurements
- SS phages are genetically enginnered by replacing 2 N-terminal amino acids of the p8 coat protein of the fd phage. AGE and zeta potential measurements show that SS phages carry at least 1/4 less net negative surface charge than fd phages. Morphology and thickness of phages are studied at different counterion concentrations (10-3, 10-2 and 10-1M) by AFM, SEM and immunofluorescence analyses. Bundles induced by CoCl2 and CaCl2 are either metallized by chemical reduction or biomineralized for apatite-like material formation. EDX spectroscopy confirms the presence of Co, P and Ca peaks in mineralized samples. Such bottom-up manufactured phage scaffolds might be applied in bioengineering studies. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Wiley-VCH Verlag
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