Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Eunjoo | - |
dc.contributor.author | Lee, Se Geun | - |
dc.contributor.author | Kim, Hyun-Chul | - |
dc.contributor.author | Lee, Sung Jun | - |
dc.contributor.author | Baek, Chul Su | - |
dc.contributor.author | Jeong, Sang Won | - |
dc.date.accessioned | 2018-01-25T01:13:37Z | - |
dc.date.available | 2018-01-25T01:13:37Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 1568-0266 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/5324 | - |
dc.description.abstract | The preparation of a synthetic receptor for multivalent protein binding by a directed immobilization of bifunctional ligands was demonstrated using pentameric C-reactive protein (CRP) and a thiolated phosphocholine-containing ligand on a gold surface. CRP consisting of five identical, noncovalently linked subunits and having five phosphocholinebinding sites on the same face was complexed with 12-mercaptododecylphosphocholine. The complexes were reacted with a gold surface, which was blocked with BSA or 2-mercaptoethanol to avoid non-specific binding. CRP binding to the molecularly imprinted monolayer was investigated by surface plasmon resonance, exhibiting high sensitivity with a detection limit as low as 1 pM (0.12 ng/mL) and binding affinity (KA ∼ 10-7-10-9 M-1) comparable to that of immobilized anti-CRP. © 2013 Bentham Science Publishers. | - |
dc.publisher | Bentham Science Publisher | - |
dc.title | Protein-Directed Immobilization of Phosphocholine Ligands on a Gold Surface for Multivalent C-Reactive Protein Binding | - |
dc.type | Article | - |
dc.identifier.doi | 10.2174/1568026611313040012 | - |
dc.identifier.scopusid | 2-s2.0-84878787498 | - |
dc.identifier.bibliographicCitation | Current Topics in Medicinal Chemistry, v.13, no.4, pp.519 - 524 | - |
dc.subject.keywordAuthor | Synthetic receptor | - |
dc.subject.keywordAuthor | multivalent protein binding | - |
dc.subject.keywordAuthor | directed immobilization | - |
dc.subject.keywordAuthor | C-reactive protein | - |
dc.subject.keywordAuthor | phosphocholine | - |
dc.subject.keywordAuthor | molecular imprinting | - |
dc.subject.keywordAuthor | surface plasmon resonance | - |
dc.citation.endPage | 524 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 519 | - |
dc.citation.title | Current Topics in Medicinal Chemistry | - |
dc.citation.volume | 13 | - |
There are no files associated with this item.