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Gene expression analysis of 3D spheroid culture of human embryonic kidney cells

Title
Gene expression analysis of 3D spheroid culture of human embryonic kidney cells
Authors
Kim, Eun JooJeon, Won Bae
DGIST Authors
Kim, Eun Joo; Jeon, Won Bae
Issue Date
2013
Citation
Toxicology and Environmental Health Sciences, 5(2), 97-106
Type
Article
Article Type
Article
Keywords
Arginyl-Glycyl-Aspartic AcidBioinformaticsCell AdhesionCell CultureCell GrowthCell ProliferationCell Strain HEK293Cell StructureDNA MicroarrayElastinElastin-Like Protein (ELP)Gene ExpressionGenomic ArrayHEK293HumanHuman CellIn Vivo-Mimic ToxicityNucleotide SequencePriority JournalRGDRNA ExtractionUnindexed Sequence
ISSN
2005-9752
Abstract
RGD (arginine-glycine-aspartic) containing elastin-like proteins (RGD-ELP), in the structure of TGPG[VGRGD (VGVPG)6]20WPC, was treated to HEK293 cells to construct in vivo-mimic 3-dimensional (3D) spheroid cells. The constructed 3D cells had increased extracellular matrices (ECMs). To identify the induced functions of 3D cells with ECMs, genomic profiles changed in 3D cells compared to the conventional 2-dimensional (2D) cells were analyzed. In HEK293 cells exposed RGD-ELP for 72 h, the total up- and down-regulated genes (824 genes) were selected to be differentially expressed genes. Further analysis by bioinformatics showed that top functions induced in 3D cells were cell proliferation, cell-to-cell signaling, cell differentiation and movement, and connective tissue development and function. We propose that the 3D cells constructed with RGD-ELP in this study could provide more in vivo-mimic responses by cell-to-cell and cell-to-ECM signaling. © 2013 Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht.
URI
http://hdl.handle.net/20.500.11750/5333
DOI
10.1007/s13530-013-0160-y
Publisher
Korean Society of Environmental Risk Assessment and Health Science
Related Researcher
Files:
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Collection:
Companion Diagnostics and Medical Technology Research Group1. Journal Articles


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