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Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution
- Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution
- Kim, Jae Young; Seo, Eun Seok; Kim, Hyun Min; Park, Ji Won; Lim, Dong Kwon; Moon, Dae Won
- DGIST Authors
- Kim, Hyun Min; Moon, Dae Won
- Issue Date
- Nature Communications, 8(1)
- Article Type
- adenine; beta cyclodextrin; cholesterol; glycerolipid; gold nanorod; monoacylglycerol; phosphorylcholine; sphinganine; sphingolipid; sphingosine; atmospheric pressure; cells and cell components; imaging method; ionization; mass spectrometry; molecular analysis; nanoparticle; spatial resolution; adult; airflow; animal tissue; Article; atmospheric pressure; confocal laser scanning microscopy; controlled study; desorption; endocytosis; environmental temperature; fluorescence imaging; hippocampal tissue; ionization; mass spectrometry; mouse; nonhuman; surface property; tissue slice; transmission electron microscopy
- We report a high spatial resolution mass spectrometry (MS) system that allows us to image live hippocampal tissue slices under open-air atmospheric pressure (AP) and ambient temperature conditions at the subcellular level. The method is based on an efficient desorption process by femtosecond (fs) laser assisted with nanoparticles and a subsequent ionization step by applying nonthermal plasma, termed AP nanoparticle and plasma assisted laser desorption ionization (AP-nanoPALDI) MS method. Combining the AP-nanoPALDI with microscopic sample scanning, MS imaging with spatial resolution of 2.9 μm was obtained. The observed AP-nanoPALDI MS imaging clearly revealed the differences of molecular composition between the apical and basal dendrite regions of a hippocampal tissue. In addition, the AP-nanoPALDI MS imaging showed the decrease of cholesterol in hippocampus by treating with methyl β-cyclodextrin, which exemplifies the potential of AP-nanoPALDI for live tissue imaging for various biomedical applications without any chemical pretreatment and/or labeling process. © 2017 The Author(s).
- Nature Publishing Group
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