Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Seong-Woon Yu | - |
| dc.contributor.author | Ji, Sora | - |
| dc.date.accessioned | 2018-03-14T02:03:32Z | - |
| dc.date.available | 2018-03-14T02:03:32Z | - |
| dc.date.issued | 2018 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000006270 | en_US |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/6030 | - |
| dc.description.abstract | The autophagy related gene 8 (Atg8) family proteins play a central role at various steps of autophagy, including autophagosome initiation, elongation, maturation and lysosome fusion, as well as cargo targeting. However, details regarding the distinct roles of individual Atg8 members during autophagy is not much known. The objective of this project is to determine the roles of each Atg8 family protein and understand their intracellular signaling and molecular effects during autophagy induced by 2-deoxy-D-glucose (2-DG) treatment in adult hippocampal neural stem (HCN) cells. Especially, we will be focusing on three proteins: microtubule-associated protein 1 light chain 3 beta (LC3B), GABA type A receptor associated protein like 1 (GABARAPL1), and 2 (GABARAPL2). HCN cells with stable knockdown of individual Atg8 family member proteins will be treated with 2-DG to induce autophagy and downstream signaling pathways will be examined. Through these experiments, we will elucidate the effects of Atg8 family proteins and further understand the relevance of Atg8 proteins during autophagy in HCN cells following 2-DG treatment. ⓒ 2017 DGIST |
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| dc.description.statementofresponsibility | open | - |
| dc.description.tableofcontents | 1. Introduction 5-- 1.1 General introduction to autophagy 5-- 1.2 Adult hippocampal neural stem cells 5-- 1.3 ATG8 family members 8-- 1.4 2-deoxy-D-glucose 12-- 2. Materials & Methods 14-- 2.1 Cell culture & Atg8 member knockdown cell 14-- 2.2 Antibody and Chemicals 14-- 2.3 Cell Death Assay 15-- 2.4 2DG drug preparation and treatment 15-- 2.5 Western Blot 16-- 3. Results 17-- 3.1 Effects of Atg8 knockdowns on HCN cell survival 17-- 3.2 Responses of Atg8 family members upon 2DH treatment 18-- 3.3 Effects of Atg8 knockdown in HCN cells upon 2DG treatment 20-- 4. Discussion 26-- Reference 30-- Korean Abstract 32 |
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| dc.format.extent | 32 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.subject | Atg8 | - |
| dc.subject | 2-deoxy-D-glucose | - |
| dc.subject | autophagy | - |
| dc.subject | hippocampal neural stem cells | - |
| dc.subject | 자가포식 | - |
| dc.subject | 성체 해마 줄기세포 | - |
| dc.title | Role of Atg8 family proteins during autophagy following 2-deoxy-D-glucose treatment in adult hippocampal neural stem cells. | - |
| dc.title.alternative | 2-deoxy-D-glucose 약물 처리 후. 성체 해마 줄기세포내 Atg8 family 단백질의 역할. | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/thesis.200000006270 | - |
| dc.description.alternativeAbstract | 자가포식 관련 유전자 8 (Atg8)은 오토파고좀 형성 개시, 신장, 성숙 뿐 아니라, 라이소좀과의 결합, 그리고 운반 표적 선정을 포함한 자가포식 과정 전반에 걸쳐 주요한 역할을 한다. 그러나, 자가포식 과정에서 개별 Atg8 단백질이 어떠한 역할을 하는지 아직 완벽히 밝혀지지는 않았다. 본 논문을 통해, 우리는 2-deoxy-D-glucose (2DG) 약물을 처리한 성체 해마 줄기세포에서 개별 Atg8 단백질의 역할 및 세포내 신호 전달과 분자 효과를 관찰하고자 한다. 특히, 우리는 쥐의 성체 해마 줄기세포에서 Atg8 family 단백질 중 세가지 단백질: LC3B, GABARAPL1, 그리고 GABARAPL2에 집중을 할 것이며, 개별 Atg8이 knockdown된 성체 해마 줄기세포에 2DG를 처리하여 자가포식을 유도한 후, 그에 따른 하위 신호 전달 경로와 관련 단백질 발현의 변화를 관찰 할 것이다. 위의 실험을 통해, 우리는 Atg8 family 단백질들의 효과 뿐 아니라, 성체 해마 줄기세포에 2DG를 처리하여 세포생존성 자가포식을 유도하였을 때, 자가포식과 관련하여 Atg8 단백질들의 추가적인 역할을 알아내고자 한다. 궁극적으로, 우리는 이 결과가 개별 Atg8 단백질의 역할에 대한 이해를 더욱 높이고, 나아가, 자가포식 관련 신경변성질환에 대한 이해에 기여 할 수 있기를 바란다. |
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| dc.description.degree | Master | - |
| dc.contributor.department | Brain and Cognitive Sciences | - |
| dc.contributor.coadvisor | Lee, Byung Hoon | - |
| dc.date.awarded | 2018. 2 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.date.accepted | 2018-01-05 | - |
| dc.contributor.alternativeDepartment | 대학원 뇌인지과학전공 | - |
| dc.contributor.alternativeName | 지소라 | - |
| dc.contributor.alternativeName | 유성운 | - |
| dc.contributor.alternativeName | 이병훈 | - |
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