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MicroRNAs as modulators of circadian gene Per2 oscillation

Title
MicroRNAs as modulators of circadian gene Per2 oscillation
Translated Title
miRNA 에 의한 생체시계 유전자 Per2 조절
Authors
Park, Inah
DGIST Authors
Park, In Ah; Kim, Kyung Jin; Youngshik Choe
Advisor(s)
Kim, Kyungjin
Co-Advisor(s)
Choe, Young Shik
Issue Date
2017
Available Date
2017-01-18
Degree Date
2017. 2
Type
Thesis
Keywords
Circadian RhythmmiR-24-3pmiR-25-3pPeriod2 geneReal-time Bioluminescence Analysis생체리듬Period2실시간 인광 분석법
Abstract
Circadian clock controls an organism’s biological rhythm and regulates physiological conditions in response to external time cues. Most of living organisms have their own time-keeping mechanism that is maintained by transcriptional-translational autoregulatory feedback loops involving several clock genes such as Periods, Cryptochromes, Clock and Bmal1. Recent discoveries have found the relevance between changes in circadian oscillation and post-transcriptional modification by microRNAs (miRNAs). However, the specific mechanisms of miRNAs on circadian oscillation still remain unclear. This study demonstrated the regulatory effects of miR-24-3p and miR-25-3p on Period2 (Per2) expression by direct interaction with 3’ untranslated region (UTR) of Per2 using luciferase reporter assay. Furthermore, real-time bioluminescence analyses demonstrated that PER2 oscillation patterns were sensitive to intracellular concentration of miR-24-3p or miR-25-3p. Elevation of either miR-24-3p or miR-25p-3p resulted phase advancing and dampening, while down regulation of miR-24-3p and miR-25-3p caused phase delay and increase in relative amplitude of PER2 oscillation. In summary, miR-24-3p and miR-25-3p are involved in fine-tuning of circadian rhythmicity through regulating PER2 oscillation in conjunction with other post-translational modulators. ⓒ 2017 DGIST
Table Of Contents
1. Introduction 7-- 2. Materials and Methods 11-- 3. Results 14-- 3.1 Functional Validation of Candidate microRNAs Targeting 3’UTR of mouse Period2 mRNA 14-- 3.2 Overexpression of miR-24-3p or miR-25-3p Downregulates PER2 Expression in Real-time Bioluminescence Analysis 15-- 3.3 Inhibition of Endogenous miR-24-3p or miR-25-3p Upregulates PER2 Expression in Real-time Bioluminescence Analysis 16-- 3.4 3’ Untranslated Region of Per2 mRNA is Essential for miR-24-3p and miR-25-3p for Modulating Circadian Oscillation 16-- 4. Discussion 38-- 5. References 42
URI
http://dgist.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002328669
http://hdl.handle.net/20.500.11750/1496
DOI
10.22677/thesis.2328669
Degree
Master
Department
Brain and Cognitive Sciences
University
DGIST
Files:
Collection:
Brain and Cognitive SciencesThesesMaster


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