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Olfactory Marker Protein Expression Is an Indicator of Olfactory Receptor-Associated Events in Non-Olfactory Tissues

Title
Olfactory Marker Protein Expression Is an Indicator of Olfactory Receptor-Associated Events in Non-Olfactory Tissues
Authors
Kang, N[Kang, NaNa]Kim, H[Kim, Hyerin]Jae, Y[Jae, YoonGyu]Lee, N[Lee, NaHye]Ku, CR[Ku, Cheol Ryong]Margolis, F[Margolis, Frank]Lee, EJ[Lee, Eun Jig]Bahk, YY[Bahk, Young Yil]Kim, MS[Kim, Min-Soo]Koo, J[Koo, JaeHyung]
DGIST Authors
Kang, N[Kang, NaNa]; Kim, H[Kim, Hyerin]; Jae, Y[Jae, YoonGyu]; Lee, N[Lee, NaHye]; Kim, MS[Kim, Min-Soo]Koo, J[Koo, JaeHyung]
Issue Date
2015-01-30
Citation
PLoS ONE, 10(1)
Type
Article
Article Type
Article
Keywords
Adenylate CyclaseAdenylate Cyclase 3Animal CellAnimal TissueCalcitoninControlled StudyCytokeratin 14Epithelium CellGlial Fibrillary Acidic ProteinGuanine Nucleotide Binding ProteinHeartHEK293 Cell LineHumanHuman CellImmunoassayImmunofluorescenceImmunohistochemistryImmunoprecipitationInterstitial Cell of CajalLeydig CellMicroarray AnalysisMouseNon-HumanOlfactory G ProteinOlfactory Marker ProteinOlfactory ReceptorProtein ExpressionReal Time Polymerase Chain ReactionReverse Transcriptase-Polymerase Chain ReactionRNA IsolationSignal TransductionStem Cell Factor ReceptorThymusThyroid Parafollicular CellUnclassified DrugWestern Blotting
ISSN
1932-6203
Abstract
Olfactory receptor (OR)-associated events are mediated by well-conserved components in the olfactory epithelium, including olfactory G-protein (Golf), adenylate cyclase III (ACIII), and olfactory marker protein (OMP). The expression of ORs has recently been observed in non-olfactory tissues where they are involved in monitoring extracellular chemical cues. The large number of OR genes and their sequence similarities illustrate the need to find an effective and simple way to detect non-olfactory OR-associated events. In addition, expression profiles and physiological functions of ORs in non-olfactory tissues are largely unknown. To overcome limitations associated with using OR as a target protein, this study used OMP with Golf and ACIII as targets to screen for potential OR-mediated sensing systems in non-olfactory tissues. Here, we show using western blotting, real-time PCR, and single as well as double immunoassays that ORs and OR-associated proteins are coexpressed in diverse tissues. The results of immunohistochemical analyses showed OMP (+) cells in mouse heart and in the following cells using the corresponding marker proteins c-kit, keratin 14, calcitonin, and GFAP in mouse tissues: interstitial cells of Cajal of the bladder, medullary thymic epithelial cells of the thymus, parafollicular cells of the thyroid, and Leydig cells of the testis. The expression of ORs in OMP (+) tissues was analyzed using a refined microarray analysis and validated with RT-PCR and real-time PCR. Three ORs (olfr544, olfr558, and olfr1386) were expressed in the OMP (+) cells of the bladder and thyroid as shown using a co-immunostaining method. Together, these results suggest that OMP is involved in the OR-mediated signal transduction cascade with olfactory canonical signaling components between the nervous and endocrine systems. The results further demonstrate that OMP immunohistochemical analysis is a useful tool for identifying expression of ORs, suggesting OMP expression is an indicator of potential OR-mediated chemoreception in non-olfactory systems. © 2015 Kang et al.
URI
http://hdl.handle.net/20.500.11750/2940
DOI
10.1371/journal.pone.0116097
Publisher
Public Library of Science
Related Researcher
Files:
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Collection:
Information and Communication EngineeringInfoLab1. Journal Articles


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