Cited 7 time in webofscience Cited 7 time in scopus

NON-CONTACT HIGH-FREQUENCY ULTRASOUND MICROBEAM STIMULATION FOR STUDYING MECHANOTRANSDUCTION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

Title
NON-CONTACT HIGH-FREQUENCY ULTRASOUND MICROBEAM STIMULATION FOR STUDYING MECHANOTRANSDUCTION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS
Authors
Hwang, JY[Hwang, Jae Youn]Lim, HG[Lim, Hae Gyun]Yoon, CW[Yoon, Chi Woo]Lam, KH[Lam, Kwok Ho]Yoon, S[Yoon, Sangpil]Lee, C[Lee, Changyang]Chiu, CT[Chiu, Chi Tat]Kang, BJ[Kang, Bong Jin]Kim, HH[Kim, Hyung Ham]Shung, KK[Shung, K. Kirk]
DGIST Authors
Hwang, JY[Hwang, Jae Youn]
Issue Date
2014-09
Citation
Ultrasound in Medicine and Biology, 40(9), 2172-2182
Type
Article
Article Type
Article
Keywords
Acoustic PressuresAcoustic StimulationAuditory StimulationCalciumCalcium Cell LevelCalcium Fluorescence ImagingCalcium SignalingCell StimulationCellular ResponseControlled StudyDevicesEchographyEndothelial CellsFluorescence ImagingFluorescence MicroscopyHigh-Energy Shock WavesHigh-Frequency Ultrasound MicrobeamHigh Frequency Ultrasound Microbeam StimulationHigh Frequency UltrasoundsHighly Focused UltrasoundsHumanHuman CellHuman Umbilical Vein Endothelial CellsHumansMechanical StimulationMechanotransductionMechanotransduction, CellularMetabolismMicroscopy, FluorescenceOptical ImagingPhysiologyPriority JournalProceduresProcedures Concerning CellsRadiationSound IntensityUltrasonicsUltrasoundUmbilical Vein Endothelial Cell
ISSN
0301-5629
Abstract
We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified. © 2014 World Federation for Ultrasound in Medicine & Biology.
URI
http://hdl.handle.net/20.500.11750/3048
DOI
10.1016/j.ultrasmedbio.2014.03.018
Publisher
Elsevier
Related Researcher
  • Author Hwang, Jae Youn MBIS(Multimodal Biomedical Imaging and System) Laboratory
  • Research Interests
Files:
There are no files associated with this item.
Collection:
Information and Communication EngineeringETC1. Journal Articles


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