Cited 12 time in webofscience Cited 17 time in scopus

Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway in lipopolysaccharide-stimulated RAW 264.7 macrophages

Title
Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway in lipopolysaccharide-stimulated RAW 264.7 macrophages
Authors
Jeong, JW[Jeong, Jin-Woo]Lee, HH[Lee, Hye Hyeon]Han, MH[Han, Min Ho]Kim, GY[Kim, Gi-Young]Hong, SH[Hong, Su Hyun]Park, C[Park, Cheol]Choi, YH[Choi, Yung Hyun]
Issue Date
2014-03-15
Citation
BMC Complementary and Alternative Medicine, 14
Type
Article
Article Type
Article
Keywords
AlcoholAnimalAnimal CellAnimalsAnti-InflammationAnti-Inflammatory ActivityAnti-Inflammatory AgentAnti-Inflammatory AgentsAutacoidBiological ProductBiological ProductsCell LineCell ViabilityCellular DistributionChemically InducedControlled StudyCyclooxygenase 2CytokineCytokine ProductionCytokine ReleaseCytokinesDinoprostoneDNA-BindingDrug EffectsEnzyme DegradationEnzyme InhibitionGel Mobility Shift AssayGenetic TranscriptionHerbal MedicineI Kappa BImmunofluorescenceImmunoglobulin Enhancer Binding ProteinImmunologyIn Vitro StudyInducible Nitric Oxide SynthaseInflammationInflammation MediatorsInterleukin-1 BetaIntracellular TransportLipopolysaccharideLipopolysaccharidesMacrophageMacrophagesMessenger RNAMetabolismMiceMouseNF-Kappa BNitric OxideNitric Oxide Synthase Type IiiNitriteNon-HumanNOS2 Protein, HumanPhytotherapyPoriaPoria CocosPoria Cocos ExtractProstaglandin E2Prostaglandin ReleaseProstaglandin SynthesisProtein DegradationProtein DNA BindingProtein ExpressionProtein TransportPTGS2 Protein, HumanRaw 264.7 CellsRaw 264.7 Macrophage Cell LineReverse Transcriptase-Polymerase Chain ReactionSignal TransductionTraditional MedicineTranscription Factor RelaTumor Necrosis Factor-AlphaWestern Blotting
ISSN
1472-6882
Abstract
Background: Poria cocos Wolf, a medicinal fungus, is widely used in traditional medicines in East Asian countries owing to its various therapeutic potentials. Although several studies have demonstrated the anti-inflammatory activity of this fungus, its underlying mechanisms have not yet been clearly defined.Methods: In the present study, we have demonstrated the anti-inflammatory effects of ethanol extract of P. cocos (EEPC) in lipopolysaccaride (LPS)-stimulated RAW 264.7 macrophages. As inflammatory parameters, the productions of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin (IL)-1β and tumor necrosis factor (TNF)-α were evaluated. We also examined the EEPC's effect on the nuclear factor-kappaB (NF-κB) signaling pathway.Results: Our results indicated that EEPC exhibits a potent inhibitory effect on NO production and inhibits PGE2 release in LPS-induced macrophages without affecting cell viability. EEPC also significantly attenuated LPS-induced secretion of inflammatory cytokines IL-1β and TNF-α. Additionally, LPS-induced expression of inducible NO synthase (iNOS), cyclooxygenase (COX)-2, IL-1β, and TNF-α was decreased by pre-treatment with EEPC at the transcriptional level. Moreover, EEPC clearly inhibited LPS-induced nuclear translocation of NF-κB p65 subunits, which correlated with EEPC's inhibitory effects on inhibitor kappaB (IκB) degradation. Moreover, EEPC clearly suppressed the LPS-induced DNA-binding activity of NF-κB, as well as the nuclear translocation of the NF-κB p65, which correlated with EEPC's inhibitory effects on inhibitor kappaB (IκB) degradation.Conclusions: Taken together, our data indicates that EEPC targets the inflammatory response of macrophages via inhibition of iNOS, COX-2, IL-1β, and TNF-α through inactivation of the NF-κB signaling pathway, supporting the pharmacological basis of P. cocos as a traditional herbal medicine for treatment of inflammation and its associated disorders. © 2014 Jeong et al.; licensee BioMed Central Ltd.
URI
http://hdl.handle.net/20.500.11750/3107
DOI
10.1186/1472-6882-14-101
Publisher
BioMed Central Ltd.
Files:
There are no files associated with this item.
Collection:
ETC1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE