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Commercial Silicon-on-Insulator (SOI) Wafers as a Versatile Substrate for Laser Desorption/Ionization Mass Spectrometry

Title
Commercial Silicon-on-Insulator (SOI) Wafers as a Versatile Substrate for Laser Desorption/Ionization Mass Spectrometry
Authors
Kim, SH[Kim, Shin Hye]Kim, J[Kim, Jeongkwon]Moon, DW[Moon, Dae Won]Han, SY[Han, Sang Yun]
DGIST Authors
Moon, DW[Moon, Dae Won]
Issue Date
2013-01
Citation
Journal of the American Society for Mass Spectrometry, 24(1), 167-170
Type
Article
Article Type
Article
Keywords
Acid AdditivesCitric AcidDesorptionDetection LimitsElectrical EquipmentFlat SurfacesHeatingInsulinIonsLaserLaser-Induced Surface HeatingLaser Desorption/IonizationLaser Desorption/Ionization Mass SpectrometriesLaser Desorption/Ionization Mass SpectrometryLaser InducedLasersLimit of DetectionMass SpectrometryModels, ChemicalPeptide AnalysisPeptide SequencingPeptidesPostsource DecaySi SurfacesSiliconSilicon-on-Insulator (SOI) WaferSilicon on Insulator WaferSilicon on Insulator WafersSilicon WafersSoi WafersSpecial SurfacesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSurface HeatingThermal ConfinementThermal ProcessTime of Flight Mass SpectrometryTwo-Dimensional (2-D)
ISSN
1044-0305
Abstract
We report here that a commercial silicon-on-insulator (SOI) wafer offers an opportunity for laser desorption/ionization (LDI) of peptide molecules, which occurs directly from its flat surface without requiring special surface preparation. The LDI-on-SOI exhibits intact ionization of peptides with a good detection limit of lower than 20 fmol, of which the mass range is demonstrated up to insulin with citric acid additives. The LDI process most likely arises from laser-induced surface heating promoted by two-dimensional thermal confinement in the thin Si surface layer of the SOI wafer. As a consequence of the thermal process, the LDI-on-SOI method is also capable of creating post-source decay (PSD) of the resulting peptide LDI ions, which is suitable for peptide sequencing using conventional TOF/TOF mass spectrometry. © 2012 American Society for Mass Spectrometry.
URI
http://hdl.handle.net/20.500.11750/3279
DOI
10.1007/s13361-012-0534-4
Publisher
Springer
Related Researcher
Files:
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Collection:
New BiologyETC1. Journal Articles


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