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Mouse neutrophils express functional umami taste receptor T1R1/T1R3

Title
Mouse neutrophils express functional umami taste receptor T1R1/T1R3
Authors
Lee, NaHyeJung, Young SuLee, Ha YoungKang, NaNaPark, Yoo JungHwang, Jae SamBahk, Young YilKoo, JaeHyungBae, Yoe-Sik
DGIST Authors
Koo, JaeHyung
Issue Date
2014-11-30
Citation
BMB Reports, 47(11), 649-654
Type
Article
Article Type
Article
Keywords
AlanineAnimalAnimalsAntagonists and InhibitorsCalciumCell MotionCell MovementChemistryChemotaxisCytokineCytokinesCytologyDrug EffectsG Protein-Coupled ReceptorGene Expression RegulationGeneticsImmunoglobulin Enhancer Binding ProteinInflammationIsolation and PurificationLipopolysaccharideLipopolysaccharidesMetabolismMiceMouseNeutrophilNeutrophilsNF-Kappa BPhosphorylationReceptors, G-Protein-CoupledRNASequence AnalysisSequence Analysis, RNASerineSignal TransductionSTAT3 ProteinSTAT3 Transcription FactorTaste Receptors, Type1Tumor Necrosis Factor-AlphaUmami Taste Receptor T1R1/T1R3
ISSN
1976-6696
Abstract
Neutrophils play an important role in the initiation of innate immunity against infection and injury. Although many different types of G-protein coupled receptors are functionally expressed in neutrophils, no reports have demonstrated functional expression of umami taste receptor in these cells. We observed that mouse neutrophils express the umami taste receptor T1R1/T1R3 through RNA sequencing and quantitative RT-PCR analysis. Stimulation of mouse neutrophils with L-alanine or L-serine, which are ligands for the umami taste receptor, elicited not only ERK or p38 MAPK phosphorylation but also chemotactic migration. Moreover, addition of L-alanine or L-serine markedly reduced the production of several cytokines including TNF-α induced by lipopolysaccharide (LPS) through inhibition of NF-κB activity or STAT3 phosphorylation in neutrophils. Our findings demonstrate that neutrophils express the umami taste receptor, through which tastants stimulate neutrophils, resulting in chemotactic migration, and attenuation of LPS-induced inflammatory response. © 2014 by the The Korean Society for Biochemistry and Molecular Biology.
URI
http://hdl.handle.net/20.500.11750/5236
DOI
10.5483/BMBRep.2014.47.11.185
Publisher
Korean Society for Biochemistry and Molecular Biology
Related Researcher
  • Author Koo, Jae Hyung The Koo Lab - ChemoReception Laboratory(CRLab)
  • Research Interests
Files:
There are no files associated with this item.
Collection:
Brain and Cognitive SciencesThe Koo Lab - ChemoReception Laboratory(CRLab)1. Journal Articles


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