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Protein-Directed Immobilization of Phosphocholine Ligands on a Gold Surface for Multivalent C-Reactive Protein Binding

Title
Protein-Directed Immobilization of Phosphocholine Ligands on a Gold Surface for Multivalent C-Reactive Protein Binding
Authors
Kim, EunjooLee, Se GeunKim, Hyun-ChulLee, Sung JunBaek, Chul SuJeong, Sang Won
DGIST Authors
Kim, EunjooLee, Se GeunKim, Hyun-ChulLee, Sung JunJeong, Sang Won
Issue Date
2013-02
Citation
Current Topics in Medicinal Chemistry, 13(4), 519-524
Type
Article
Article Type
Review
Keywords
12 MercaptododecylphosphocholineBinding AffinityBinding SitesC-Reactive ProteinC Reactive ProteinColumn ChromatographyControlled StudyDirected ImmobilizationGoldHumanHumansLigandsMercaptoethanolMolecular ImprintingMonolayer CultureMultivalent Protein BindingPhosphocholinePhosphorylcholineProtein BindingProtein ImmobilizationProtein SynthesisSurface Plasmon ResonanceSurface PropertiesSynthetic ReceptorUnclassified Drug
ISSN
1568-0266
Abstract
The preparation of a synthetic receptor for multivalent protein binding by a directed immobilization of bifunctional ligands was demonstrated using pentameric C-reactive protein (CRP) and a thiolated phosphocholine-containing ligand on a gold surface. CRP consisting of five identical, noncovalently linked subunits and having five phosphocholinebinding sites on the same face was complexed with 12-mercaptododecylphosphocholine. The complexes were reacted with a gold surface, which was blocked with BSA or 2-mercaptoethanol to avoid non-specific binding. CRP binding to the molecularly imprinted monolayer was investigated by surface plasmon resonance, exhibiting high sensitivity with a detection limit as low as 1 pM (0.12 ng/mL) and binding affinity (KA ∼ 10-7-10-9 M-1) comparable to that of immobilized anti-CRP. © 2013 Bentham Science Publishers.
URI
http://hdl.handle.net/20.500.11750/5324
DOI
10.2174/1568026611313040012
Publisher
Bentham Science Publisher
Related Researcher
Files:
There are no files associated with this item.
Collection:
Companion Diagnostics and Medical Technology Research Group1. Journal Articles
Magnet-Controlled Materials Research Group1. Journal Articles


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