Cited 23 time in webofscience Cited 25 time in scopus

Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells

Title
Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells
Authors
Han, SeungminFink, JuergenJorg, David J.Lee, EunminYum, Min KyuChatzeli, LemoniaMerker, Sebastian R.Josserand, ManonTrendafilova, TeodoraAndersson-Rolf, AmandaDabrowska, CatherineKim, HyunkiNaumann, RonaldLee, Ji-HyunSasaki, NobuoRichard Lester MortBasak, OnurClevers, HansStange, Danie E.Philpott, AnnaKim, Jong KyoungSimons, Benjamin D.Koo, Bon-Kyoung
DGIST Authors
Lee, Eunmin; Kim, Jong Kyoung
Issue Date
2019-09
Citation
Cell Stem Cell, 25(3), 342-356.e7
Type
Article
Article Type
Article
Author Keywords
Troyintestinegastric corpus isthmus stem celltwo stem cell compartmentspunctuated neutral driftunbiased genetic labelingdeep tissue imagingbiophysical modelingsingle-cell RNA-seqLgr5
Keywords
EPITHELIAL-CELLSMOUSE STOMACHCHIEF CELLSRNA-SEQOXYNTIC ATROPHYCORPUSLINEAGEIDENTIFICATIONREGENERATIONEXPRESSION
ISSN
1934-5909
Abstract
The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of “punctuated” neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs. © 2019 The Authors
URI
http://hdl.handle.net/20.500.11750/10444
DOI
10.1016/j.stem.2019.07.008
Publisher
Cell Press
Related Researcher
  • Author Kim, Jong Kyoung Laboratory of Single-Cell Genomics
  • Research Interests Single-cell genomics, Bioinformatics, Machine Learning
Files:
There are no files associated with this item.
Collection:
Department of New BiologyETC1. Journal Articles
Department of New BiologyLaboratory of Single-Cell Genomics1. Journal Articles


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