Cited 2 time in webofscience Cited 2 time in scopus

Immunochromatographic assay to detect alpha-tubulin in urine for the diagnosis of kidney injury

Title
Immunochromatographic assay to detect alpha-tubulin in urine for the diagnosis of kidney injury
Authors
Choi, Eun-SookFaruque, Md Hasan AlKim, Jung-HeeCho, Jang-HeePark, Kwon MooKim, Eunjoo
DGIST Authors
Choi, Eun-Sook; Faruque, Md Hasan Al; Kim, Jung-Hee; Cho, Jang-Hee; Park, Kwon Moo; Kim, Eunjoo
Issue Date
2020-01
Citation
Journal of Clinical Laboratory Analysis, 34(1)
Type
Article
Article Type
Article; Early Access
Author Keywords
cellulose nanobeadsimmunochromatographykidney injuryrapid kitalpha-tubulin
Keywords
PRIMARY CILIA LENGTHISCHEMIA/REPERFUSION INJURYRAPID DETECTION
ISSN
0887-8013
Abstract
Backgrounds: Shortening of primary cilia in kidney epithelial cells is associated with kidney injury and involved with the induced level of α-tubulin in urine. Therefore, rapid detection and quantification of α-tubulin in the urine samples could be used to the preliminary diagnosis of kidney injury. Methods: Cellulose-based nanobeads modified with α-tubulin were used for the detection probe of competitive immunochromatographic (IC) assay. The concentration of α-tubulin in the urine samples was determined by IC assay and compared with the amount determined by Western blotting analysis. Results: The relationship between α-tubulin concentration and the colorimetric intensity resulted from IC assay was determined by logistic regression, and the correlation coefficient (R2) was 0.9948. When compared to the amount determined by Western blotting analysis, there was a linear relationship between the α-tubulin concentrations measured by the two methods and the R2 value was 0.823. Conclusions: This method is simple, rapid, and adequately sensitive to detect α-tubulin in patient urine samples, which could be used for the clinical diagnosis of kidney injury. © 2019 Wiley Periodicals, Inc.
URI
http://hdl.handle.net/20.500.11750/10657
DOI
10.1002/jcla.23015
Publisher
John Wiley & Sons Inc.
Related Researcher
  • Author Kim, Eunjoo  
  • Research Interests Biomarker, liquid biopsy, molecular diagnosis, nanobiosensor, drug delivery, exosome
Files:
There are no files associated with this item.
Collection:
Division of Electronics & Information System1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE