Title

Study on long noncoding RNAs-mediated temporal control in Arabidopsis leaf senescence

DGIST Authors

TRANG, TRAN THI MY ; Lim, Pyung Ok ; Lee, Young-Sam

Advisor

임평옥

Co-Advisor(s)

Young-Sam Lee

Issued Date

2019

Awarded Date

2019-02

Citation

TRAN THI MY TRANG. (2019). Study on long noncoding RNAs-mediated temporal control in Arabidopsis leaf senescence. doi: 10.22677/thesis.200000171536

Type

Thesis

Table Of Contents

Abstract i
List of content ii
List of Tables and figures iv
Abbreviations v
I. INTRODUCTION 1
1.1. Introduction of leaf senescence 1
1.2. Introduction of long noncoding RNA (lncRNA) 1
1.3. Classification of lncRNA in genomic context 2
1.3.1. Characteristics of antisense lncRNA 2
1.3.2. Characteristics of lincRNA 3
1.4. Structural features of lncRNAs 3
1.5. Regulation of lncRNAs 5
1.5.1. Regulation of lncRNA in the nucleus 5
1.5.2. Regulation of lncRNA in the cytoplasm 6
1.6. Objectives 8
II. MATERIALS AND METHODS 9
2.1. Plant Materials and Growth Conditions. 9
2.2. Age/Stress- induced leaf senescence assay. 9
2.3. RNA isolation and gene expression analysis 9
III. RESULTS 10
3.1. Identification of senescence-associated lncRNAs (SALNRs) in Arabidopsis 10
3.1.1. Selection of SALNRs from RNA-seq database based on their expression 10
3.1.2. Isolation of knockout mutants of SALNRs. 10
3.1.4. Examination of the senescence phenotype (age, stress-induced senescence) of SALNRs in leaf yellowing phenotype and Fv/Fm ratio compared with Col-0 12
3.1.5. Selection of SALNR4, SALNR5 for further study 14
3.2. Characterization of the function of SALNR4, SALNR5 18
3.2.1. Confirmation of the senescence phenotype of salnr4, salnr5 mutants 18
ⅠV. DISCUSSION AND CONCLUSION 27
4.1. Discussion 27
4.2. Conclusion 30
References 31
Summary 38
Acknowledgement 40
Curriculum Vitae 41

URI

http://dgist.dcollection.net/common/orgView/200000171536
http://hdl.handle.net/20.500.11750/10749

DOI

10.22677/thesis.200000171536

Degree

MASTER

Department

New Biology

Publisher

DGIST

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