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Common Repository of FBS Proteins (cRFP) to Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome

Title
Common Repository of FBS Proteins (cRFP) to Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome
Authors
Shin, JihyeKwon, YumiLee, SeonjeongNa, SeungjinHong, Eun YoungJu, ShinyeongJung, Hyun-GyoKaushal, PrashantShin, SunghoBack, Ji HyunChoi, Seon YoungKim, Eun HeeLee, Su JinPark, Yae EunAhn, Hee-SungAhn, YoungheeKabir, Mohammad HumayunPark, Seong-JunYang, Won SukYeom, JeonghunBang, Oh YoungHa, Chul-WonLee, Jin-WonKang, Un-BeomKim, Hye-JungPark, Kang-SikLee, J. EugeneLee, Ji EunKim, Jin YoungKim, Kwang PyoKim, YoungsooHirano, HisashiYi, Eugene C.Cho, Je-YoelPaek, EunokLee, Cheolju
Issue Date
2019-10
Citation
Journal of Proteome Research, 18(10), 3800-3806
Type
Article
Article Type
Article
Author Keywords
secretomecommon repository of FBS proteinsdatabase search
Keywords
NEWLY SYNTHESIZED PROTEINSSERUM
ISSN
1535-3893
Abstract
We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretomes. cRFP is a small supplementary sequence list of highly abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the contaminant FBS proteins from being misidentified as other proteins in the reference database, just as we would use cRAP (common Repository of Adventitious Proteins) to prevent contaminant proteins present either by accident or through unavoidable contacts from being misidentified as other proteins. We expect it to be widely used in experiments where the proteins are obtained from serum-free media after thorough washing of the cells, or from a complex media such as SILAC, or from extracellular vesicles directly. Copyright © 2019 American Chemical Society.
URI
http://hdl.handle.net/20.500.11750/10843
DOI
10.1021/acs.jproteome.9b00475
Publisher
American Chemical Society
Files:
There are no files associated with this item.
Collection:
ETC1. Journal Articles


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