Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Seung Joon | ko |
dc.contributor.author | Sim, Tae Seok | ko |
dc.contributor.author | Shin, Hyun Young | ko |
dc.contributor.author | Lee, Jeong Min | ko |
dc.contributor.author | Kim, Min. Young. | ko |
dc.contributor.author | Sunoo, Joseph | ko |
dc.contributor.author | Lee, Jeong-Gun | ko |
dc.contributor.author | Yea, Kyungmoo | ko |
dc.contributor.author | Kim, Young Zoon | ko |
dc.contributor.author | Van Noort, D. | ko |
dc.contributor.author | Park, Soo Kyung | ko |
dc.contributor.author | Kim, Woon-Hae | ko |
dc.contributor.author | Park, Kyun Woo | ko |
dc.contributor.author | Kim, Minseok S. | ko |
dc.date.accessioned | 2019-12-16T01:02:30Z | - |
dc.date.available | 2019-12-16T01:02:30Z | - |
dc.date.created | 2019-11-07 | - |
dc.date.issued | 2019-10 | - |
dc.identifier.citation | PLoS ONE, v.14, no.10, pp.e0223193 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/10981 | - |
dc.description.abstract | Microchips are widely used to separate circulating tumor cells (CTCs) from whole blood by virtues of sophisticated manipulation for microparticles. Here, we present a chip with an 8 μm high and 27.9 mm wide slit to capture cancer cells bound to 3 μm beads. Apart from a higher purity and recovery rate, the slit design allows for simplified fabrication, easy cell imaging, less clogging, lower chamber pressure and, therefore, higher throughput. The beads were conjugated with anti-epithelial cell adhesion molecules (anti-EpCAM) to selectively bind to breast cancer cells (MCF-7) used to spike the whole blood. The diameter of the cell-bead construct was in average 23.1 μm, making them separable from other cells in the blood. As a result, the cancer cells were separated from 5 mL of whole blood with a purity of 52.0% and a recovery rate of 91.1%, and also we confirmed that the device can be applicable to clinical samples of human breast cancer patients. The simple design with microslit, by eliminating any high-aspect ratio features, is expected to reduce possible defects on the chip and, therefore, more suitable for mass production without false separation outputs. © 2019 Lee et al. | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.title | Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/journal.pone.0223193 | - |
dc.identifier.wosid | 000520458300001 | - |
dc.identifier.scopusid | 2-s2.0-85074059757 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.contributor.nonIdAuthor | Sim, Tae Seok | - |
dc.contributor.nonIdAuthor | Sunoo, Joseph | - |
dc.contributor.nonIdAuthor | Lee, Jeong-Gun | - |
dc.contributor.nonIdAuthor | Kim, Young Zoon | - |
dc.contributor.nonIdAuthor | Van Noort, D. | - |
dc.contributor.nonIdAuthor | Park, Soo Kyung | - |
dc.contributor.nonIdAuthor | Park, Kyun Woo | - |
dc.identifier.citationVolume | 14 | - |
dc.identifier.citationNumber | 10 | - |
dc.identifier.citationStartPage | e0223193 | - |
dc.identifier.citationTitle | PLoS ONE | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | SIZE | - |
dc.subject.keywordPlus | POPULATIONS | - |
dc.subject.keywordPlus | BIOMARKER | - |
dc.subject.keywordPlus | METASTATIC BREAST-CANCER | - |
dc.subject.keywordPlus | ISOLATION PLATFORM | - |
dc.subject.keywordPlus | EFFICIENT CAPTURE | - |
dc.subject.keywordPlus | PERIPHERAL-BLOOD | - |
dc.contributor.affiliatedAuthor | Yea, Kyungmoo | - |
dc.contributor.affiliatedAuthor | Kim, Woon-Hae | - |
dc.contributor.affiliatedAuthor | Kim, Minseok S. | - |