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Simultaneous Determination of Seven Sesquiterpene Lactone Glucosides in Ixeris dentata by High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and their Antiviral Activities

Title
Simultaneous Determination of Seven Sesquiterpene Lactone Glucosides in Ixeris dentata by High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and their Antiviral Activities
Authors
Park, SeonJuKim, NanyoungPark, Jun HyungLee, Sang-WonSong, Jae-HyoungKo, Hyun-JeongChae, Han-JungKim, Hyoung-RyougKim, Seung Hyun
DGIST Authors
Kim, Hyoung-Ryoug
Issue Date
2019-12
Citation
Acta Chromatographica, 31(4), 280-285
Type
Article
Article Type
Article
Author Keywords
Ixeris dentataguaianolidesgermacranolideHPLC-MS/MSantiviral activity
Keywords
SONCHIFOLIAINFECTIONALBIFLORA
ISSN
1233-2356
Abstract
Ixeris dentata (Thunb. ex Thunb.) Nakai (Asteraceae), a well-known edible vegetable in Asia, contains various bioactive secondary metabolites, including sesquiterpene lactones. In this study, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed and validated for simultaneous determination of seven sesquiterpene lactone glucosides isolated from the roots of I. dentata. In addition, these compounds were evaluated in terms of their antiviral activities against coxsackievirus B3 (CVB3) and human enterovirus 71 (EV71). The developed method was validated in terms of linearity (R-2 > 0.9996), precision (RSD < 2.24%), accuracy (96.30-102.77%), and stability (RSD < 1.94%) and successfully applied to the quantitation of the I. dentata root samples collected from six different regions of Korea. The content of sesquiterpene lactone glucosides varied significantly based on the region. For the antiviral activities, guaianolides with an ester group at C-8 (compounds 6 and 7) showed the most potent activities against CBV3, while germacranolide (compound 5) showed the most consistent antiviral activity against both CVB3 and EV71. The method was validated to be simple and reliable to simultaneously determine seven putative bioactive sesquiterpene lactone glucosides, the substantial chemotaxonomic markers, in I. dentata root samples.
URI
http://hdl.handle.net/20.500.11750/11017
DOI
10.1556/1326.2018.00470
Publisher
Akademiai Kiado Rt.
Files:
Collection:
ETC1. Journal Articles


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