An apparatus and method for determining bio-information of a target using an impulse radar are provided. The method may include generating a frame set by accumulating frames received at preset time intervals, determining a first magnitude spectrum of the frame set corresponding to a first frequency axis by performing a frequency conversion of frames included in the frame set in a sampler index axis direction, determining a second magnitude spectrum of the frame set corresponding to the first frequency axis and a second frequency axis by performing a frequency conversion of the first magnitude spectrum in a time axis direction, determining a third magnitude spectrum of the frame set by adding up values of the second magnitude spectrum for each second frequency, and determining a frequency indicating a peak in the third magnitude spectrum as a heartbeat frequency of the target.