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Nature-Inspired Adhesive Catecholamines for Highly Concentrated Colorimetric Signal in Spatial Biomarker Labeling

Title
Nature-Inspired Adhesive Catecholamines for Highly Concentrated Colorimetric Signal in Spatial Biomarker Labeling
Authors
Kim, SeunghwiHong, Seonki
DGIST Authors
Kim, Seunghwi; Hong, Seonki
Issue Date
2020-08
Citation
Advanced Healthcare Materials, 9(16), 2000540
Type
Article
Article Type
Article in press
Author Keywords
adhesive colorantcatecholaminecolorimetric bioassaysmolecular probeorganic colorant
Keywords
ASSAYCOATINGS
ISSN
2192-2640
Abstract
Colorants have been utilized for precise biomarker detection in rapid and convenient colorimetric bioassays. However, the diffusion of colorants in solution often results in poor sensitivity, which is a major obstacle to the clinical translation of current colorants. To address this issue, in the current study, a unique colorant is developed that possesses adhesiveness for concentration near the target biomarker, avoiding diffusion. In nature, the synergistic interplay between catechol and amine functional groups is thought to be key for the unique mechanism of marine mussel adhesion. In addition, polymerized catecholamines are found in nature as biopigments, that is, in melanin. The dual role of catechol/catecholamine moieties in natural organics inspire to design novel colorimetric bioassays based on an adhesive colorant. Horseradish peroxidase (HRP) is used to initiate in situ polymerization of the catecholic precursors with amine-containing additive molecules and simultaneously attach them near the HRP-labeled biomarkers. This novel catecholamine-based adhesive colorant provides an excellent quantitative (naked-eye) visible signal and it also generates superb spatial information on the biomarkers on complex surfaces (e.g., cell membranes). © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
URI
http://hdl.handle.net/20.500.11750/12088
DOI
10.1002/adhm.202000540
Publisher
Wiley-VCH Verlag
Related Researcher
  • Author Hong, Seonki Biomaterials & Biointerface Engineering Laboratory
  • Research Interests Bio-inspired organic materials; Polymeric biomaterials; Surface biofunctionalization; biochip fabrication
Files:
There are no files associated with this item.
Collection:
Department of Emerging Materials ScienceBiomaterials & Biointerface Engineering Laboratory1. Journal Articles


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