Cited 13 time in webofscience Cited 13 time in scopus

Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells

Title
Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells
Authors
Lee, MinjiLee, Eun MinHan, Seong KyuChoi, Yoon HaKwon, Dong-ilChoi, HyobeenLee, KwanghwanPark, Eun SeoRha, Min-SeokJoo, Dong JinShin, Eui-CheolKim, SangukKim, Jong KyoungLee, You Jeong
DGIST Authors
Lee, Eun Min; Kim, Jong Kyoung
Issue Date
2020-08
Citation
Nature Communications, 11(1), 4367
Type
Article
Article Type
Article
Keywords
GAMMA-DELTA TSEQ DATADIVERSITYRECOGNITIONREPERTOIREREARRANGEMENTEXPRESSIONMOLECULESIMMUNITYGATA-3
ISSN
2041-1723
Abstract
Invariant natural killer T (iNKT), mucosal-associated invariant T (MAIT), and γδ T cells are innate T cells that acquire memory phenotype in the thymus and share similar biological characteristics. However, how their effector differentiation is developmentally regulated is still unclear. Here, we identify analogous effector subsets of these three innate T cell types in the thymus that share transcriptional profiles. Using single-cell RNA sequencing, we show that iNKT, MAIT and γδ T cells mature via shared, branched differentiation rather than linear maturation or TCR-mediated instruction. Simultaneous TCR clonotyping analysis reveals that thymic maturation of all three types is accompanied by clonal selection and expansion. Analyses of mice deficient of TBET, GATA3 or RORγt and additional in vivo experiments corroborate the predicted differentiation paths, while human innate T cells from liver samples display similar features. Collectively, our data indicate that innate T cells share effector differentiation processes in the thymus. © 2020, The Author(s).
URI
http://hdl.handle.net/20.500.11750/12693
DOI
10.1038/s41467-020-18155-8
Publisher
Nature Research
Related Researcher
  • Author Kim, Jong Kyoung Laboratory of Single-Cell Genomics
  • Research Interests Single-cell genomics, Bioinformatics, Machine Learning
Files:
Collection:
Department of New BiologyETC1. Journal Articles
Department of New BiologyLaboratory of Single-Cell Genomics1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE