Cited 11 time in webofscience Cited 13 time in scopus

Highly sensitive electrochemical biosensor based on naturally reduced rGO/Au nanocomposite for the detection of miRNA-122 biomarker

Title
Highly sensitive electrochemical biosensor based on naturally reduced rGO/Au nanocomposite for the detection of miRNA-122 biomarker
Authors
Kasturi, SatishEom, Yun JiTorati, Sri RamuluKim, CheolGi
DGIST Authors
Kasturi, Satish; Eom, Yun Ji; Torati, Sri Ramulu; Kim, CheolGi
Issue Date
2021-01
Citation
Journal of Industrial and Engineering Chemistry, 93, 186-195
Type
Article
Article Type
Article
Author Keywords
SoapnutmicroRNADetectionBiosensorImmobilizationNanocomposite
Keywords
GRAPHENE OXIDEGOLD NANOPARTICLESMODIFIED ELECTRODEFACILE SYNTHESISMICRORNASREDUCTIONPROBEACETAMINOPHENEXPRESSIONGRAPHITE
ISSN
1226-086X
Abstract
Herein, we have developed a novel high-resolution electrochemical biosensor for the detection of the microRNA-122 (miRNA-122) using gold nanoparticles dotted reduced graphene oxide (rGO/Au) nanocomposite. The natural soapnut solution was used as a reducing agent for the synthesis of the nanocomposite. The naturally reduced rGO/Au nanocomposite was confirmed through various characterization techniques. When rGO/Au nanocomposite was coated on to the gold electrode and checked the electrochemical performance, the nanocomposite shows superior analytical performance. The probe DNA was anchored onto the binding sites of rGO/Au nanocomposite through thiol linker and recognized the target miRNA-122. The developed rGO/Au based electrochemical biosensor demonstrated a linear response for various target miRNA-122 concentrations with a range from 10 μM to 10 pM and, with a detection limit of 1.73 pM. The developed biosensor also shows good stability and reproducibility, could be used for the detection of miRNA-122 and, also can be used for the basic research and clinical studies. Besides, the demonstrated rGO/Au nanocomposite-based sensing strategy could be used to detect various miRNA and protein biomarkers. Furthermore, the green synthesis approach could also be useful for the synthesis of various nanomaterials and nanocomposites for various biomedical applications. © 2020 The Korean Society of Industrial and Engineering Chemistry
URI
http://hdl.handle.net/20.500.11750/12789
DOI
10.1016/j.jiec.2020.09.022
Publisher
한국공업화학회
Related Researcher
  • Author Kim, CheolGi Lab for NanoBio-MatErials & SpinTronics(nBEST)
  • Research Interests Magnetic Materials and Spintronics; Converging Technology of Nanomaterials and Biomaterials; Bio-NEMS;MEMS
Files:
There are no files associated with this item.
Collection:
Department of Emerging Materials ScienceLab for NanoBio-Materials & SpinTronics(nBEST)1. Journal Articles


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