Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Lee, Shinrye | - |
dc.contributor.author | Kim, Seyeon | - |
dc.contributor.author | Kang, Ha-Young | - |
dc.contributor.author | Lim, Hye Ryeong | - |
dc.contributor.author | Baik, Seungyeob | - |
dc.contributor.author | Jo, Myungjin | - |
dc.contributor.author | Jeon, Yu-Mi | - |
dc.contributor.author | Kim, Sang Ryong | - |
dc.contributor.author | Kim, Kiyoung | - |
dc.contributor.author | Ha, Chang Man | - |
dc.contributor.author | Lee, Seongsoo | - |
dc.contributor.author | Kim, Hyung-Jun | - |
dc.date.accessioned | 2021-01-22T07:55:16Z | - |
dc.date.available | 2021-01-22T07:55:16Z | - |
dc.date.created | 2020-10-29 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.citation | Journal of Neuroinflammation, v.17, no.1, pp.299 | - |
dc.identifier.issn | 1742-2094 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/12831 | - |
dc.description.abstract | Background: Cytoplasmic inclusions of transactive response DNA binding protein of 43 kDa (TDP-43) in neurons and astrocytes are a feature of some neurodegenerative diseases, such as frontotemporal lobar degeneration with TDP-43 (FTLD-TDP) and amyotrophic lateral sclerosis (ALS). However, the role of TDP-43 in astrocyte pathology remains largely unknown. Methods: To investigate whether TDP-43 overexpression in primary astrocytes could induce inflammation, we transfected primary astrocytes with plasmids encoding Gfp or TDP-43-Gfp. The inflammatory response and upregulation of PTP1B in transfected cells were examined using quantitative RT-PCR and immunoblot analysis. Neurotoxicity was analysed in a transwell coculture system of primary cortical neurons with astrocytes and cultured neurons treated with astrocyte-conditioned medium (ACM). We also examined the lifespan, performed climbing assays and analysed immunohistochemical data in pan-glial TDP-43-expressing flies in the presence or absence of a Ptp61f RNAi transgene. Results: PTP1B inhibition suppressed TDP-43-induced secretion of inflammatory cytokines (interleukin 1 beta (IL-1β), interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF-α)) in primary astrocytes. Using a neuron-astrocyte coculture system and astrocyte-conditioned media treatment, we demonstrated that PTP1B inhibition attenuated neuronal death and mitochondrial dysfunction caused by overexpression of TDP-43 in astrocytes. In addition, neuromuscular junction (NMJ) defects, a shortened lifespan, inflammation and climbing defects caused by pan-glial overexpression of TDP-43 were significantly rescued by downregulation of ptp61f (the Drosophila homologue of PTP1B) in flies. Conclusions: These results indicate that PTP1B inhibition mitigates the neuronal toxicity caused by TDP-43-induced inflammation in mammalian astrocytes and Drosophila glial cells. © 2020, The Author(s). | - |
dc.language | English | - |
dc.publisher | BioMed Central Ltd | - |
dc.title | The overexpression of TDP-43 in astrocytes causes neurodegeneration via a PTP1B-mediated inflammatory response | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s12974-020-01963-6 | - |
dc.identifier.wosid | 000581731200001 | - |
dc.identifier.scopusid | 2-s2.0-85092663441 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.citation.publicationname | Journal of Neuroinflammation | - |
dc.contributor.nonIdAuthor | Lee, Shinrye | - |
dc.contributor.nonIdAuthor | Kim, Seyeon | - |
dc.contributor.nonIdAuthor | Kang, Ha-Young | - |
dc.contributor.nonIdAuthor | Lim, Hye Ryeong | - |
dc.contributor.nonIdAuthor | Baik, Seungyeob | - |
dc.contributor.nonIdAuthor | Jo, Myungjin | - |
dc.contributor.nonIdAuthor | Jeon, Yu-Mi | - |
dc.contributor.nonIdAuthor | Kim, Sang Ryong | - |
dc.contributor.nonIdAuthor | Kim, Kiyoung | - |
dc.contributor.nonIdAuthor | Ha, Chang Man | - |
dc.contributor.nonIdAuthor | Lee, Seongsoo | - |
dc.contributor.nonIdAuthor | Kim, Hyung-Jun | - |
dc.identifier.citationVolume | 17 | - |
dc.identifier.citationNumber | 1 | - |
dc.identifier.citationStartPage | 299 | - |
dc.identifier.citationTitle | Journal of Neuroinflammation | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordAuthor | Tar DNA-binding protein 43 | - |
dc.subject.keywordAuthor | Astrocytes | - |
dc.subject.keywordAuthor | Neurodegenerative disease | - |
dc.subject.keywordAuthor | Neuroinflammation | - |
dc.subject.keywordAuthor | Protein tyrosine phosphatase 1B | - |
dc.subject.keywordPlus | AMYOTROPHIC-LATERAL-SCLEROSIS | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | DROSOPHILA MODEL | - |
dc.subject.keywordPlus | MOTOR-NEURONS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | MITOCHONDRIAL DYSFUNCTION | - |
dc.subject.keywordPlus | BRAIN-DAMAGE | - |
dc.subject.keywordPlus | CELL-LINES | - |
dc.subject.keywordPlus | ALS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.contributor.affiliatedAuthor | Lee, Shinrye | - |
dc.contributor.affiliatedAuthor | Kim, Seyeon | - |
dc.contributor.affiliatedAuthor | Kang, Ha-Young | - |
dc.contributor.affiliatedAuthor | Lim, Hye Ryeong | - |
dc.contributor.affiliatedAuthor | Baik, Seungyeob | - |
dc.contributor.affiliatedAuthor | Jo, Myungjin | - |
dc.contributor.affiliatedAuthor | Jeon, Yu-Mi | - |
dc.contributor.affiliatedAuthor | Kim, Sang Ryong | - |
dc.contributor.affiliatedAuthor | Kim, Kiyoung | - |
dc.contributor.affiliatedAuthor | Ha, Chang Man | - |
dc.contributor.affiliatedAuthor | Lee, Seongsoo | - |
dc.contributor.affiliatedAuthor | Kim, Hyung-Jun | - |