Cited 1 time in webofscience Cited 1 time in scopus

Altered presynaptic function and number of mitochondria in the medial prefrontal cortex of adult Cyfip2 heterozygous mice

Title
Altered presynaptic function and number of mitochondria in the medial prefrontal cortex of adult Cyfip2 heterozygous mice
Authors
Kim, Gyu HyunZhang, YinhuaKang, Hyae RimLee, Seung-HyunShin, JiwonLee, Chan HeeKang, HyojinMa, RuiyingJin, ChunmeiKim, YoonheeKim, Su YeonKwon, Seok-KyuChoi, Se-YoungLee, Kea JooHan, Kihoon
DGIST Authors
Kim, Gyu Hyun; Zhang, Yinhua; Kang, Hyae Rim; Lee, Seung-Hyun; Shin, Jiwon; Lee, Chan Hee; Kang, Hyojin; Ma, Ruiying; Jin, Chunmei; Kim, Yoonhee; Kim, Su Yeon; Kwon, Seok-Kyu; Choi, Se-Young; Lee, Kea Joo; Han, Kihoon
Issue Date
2020-09
Citation
Molecular Brain, 13(1), 123
Type
Article
Article Type
Article
Author Keywords
Medial prefrontal cortexCytoplasmic FMR1-interacting protein 2PresynapseMitochondria
Keywords
CYFIP2DENSITY
ISSN
1756-6606
Abstract
Variants of the cytoplasmic FMR1-interacting protein (CYFIP) gene family, CYFIP1 and CYFIP2, are associated with numerous neurodevelopmental and neuropsychiatric disorders. According to several studies, CYFIP1 regulates the development and function of both pre- and post-synapses in neurons. Furthermore, various studies have evaluated CYFIP2 functions in the postsynaptic compartment, such as regulating dendritic spine morphology; however, no study has evaluated whether and how CYFIP2 affects presynaptic functions. To address this issue, in this study, we have focused on the presynapses of layer 5 neurons of the medial prefrontal cortex (mPFC) in adult Cyfip2 heterozygous (Cyfip2 +/- ) mice. Electrophysiological analyses revealed an enhancement in the presynaptic short-term plasticity induced by high-frequency stimuli in Cyfip2 +/- neurons compared with wild-type neurons. Since presynaptic mitochondria play an important role in buffering presynaptic Ca2+, which is directly associated with the short-term plasticity, we analyzed presynaptic mitochondria using electron microscopic images of the mPFC. Compared with wild-type mice, the number, but not the volume or cristae density, of mitochondria in both presynaptic boutons and axonal processes in the mPFC layer 5 of Cyfip2 +/- mice was reduced. Consistent with an identification of mitochondrial proteins in a previously established CYFIP2 interactome, CYFIP2 was detected in a biochemically enriched mitochondrial fraction of the mouse mPFC. Collectively, these results suggest roles for CYFIP2 in regulating presynaptic functions, which may involve presynaptic mitochondrial changes. © 2020 The Author(s).
URI
http://hdl.handle.net/20.500.11750/12838
DOI
10.1186/s13041-020-00668-4
Publisher
BioMed Central
Files:
Collection:


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE