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Microvalve-controlled miniaturized electrochemical lab-on-a-chip based biosensor for the detection of β-amyloid biomarker

Title
Microvalve-controlled miniaturized electrochemical lab-on-a-chip based biosensor for the detection of β-amyloid biomarker
Author(s)
Kasturi, SatishTorati, Sri RamuluEom, YunjiKim, CheolGi
DGIST Authors
Kasturi, SatishTorati, Sri RamuluEom, YunjiKim, CheolGi
Issued Date
2021-05
Type
Article
Author Keywords
Alzheimer&aposs diseaseBiosensorLab-on-a-chipMicrofluidic channel and valveβ-Amyloid (1–42) biomarker
Keywords
Controlled manipulationsElectrochemical biosensorEnhanced detection limitsAntigensBiosensorsCerebrospinal fluidGold microelectrodesLabel-free detectionMicrofluidic biosensorsMicrofluidic channelSpecific immobilizationBiomarkersGlycoproteinsMicroelectrodesMicrofluidicsNeurodegenerative diseasesProteinsReagents
ISSN
1226-086X
Abstract
Recently, miniaturized electrochemical biosensors have the advantage of real-time monitoring and label-free detection of biomarkers. However, controlled manipulation of reagent samples for specific immobilization of biomarkers remains a challenge to attend high-resolution microfluidic biosensor. Here, we designed a microfluidic channel and valves integrated electrochemical biosensor for the detection of β-amyloid (1–42) biomarker, one of the most neurotoxic peptides present in the cerebrospinal fluid, and a possible marker for the detection of Alzheimer's disease. The formation of the antibody-antigen complexes on gold microelectrode integrated inside the fluidic chambers was presented in the developed biosensor, and all the measurements are carefully controlled using pneumatic valves integrated with the device. The demonstrated microfluidic electrochemical biosensor exhibits a linear response towards various β-amyloid antigen concentrations from 2.2 pM to 22 μM, with an enhanced detection limit of 1.62 pM. The developed technology offers an advantage of minimal reagent sample and suitable environment for miniaturized and sensitive detection of biomarkers, could also be used for the detection of other biomarkers with high efficiency. © 2021 The Korean Society of Industrial and Engineering Chemistry
URI
http://hdl.handle.net/20.500.11750/15316
DOI
10.1016/j.jiec.2021.02.020
Publisher
한국공업화학회
Related Researcher
  • 김철기 Kim, CheolGi 화학물리학과
  • Research Interests Magnetic Materials and Spintronics; Converging Technology of Nanomaterials and Biomaterials; Bio-NEMS;MEMS
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Appears in Collections:
Department of Physics and Chemistry Lab for NanoBio-Materials & SpinTronics(nBEST) 1. Journal Articles

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