Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Topin, Jérémie | - |
dc.contributor.author | Bouysset, Cédric | - |
dc.contributor.author | Pacalon, Jody | - |
dc.contributor.author | Kim, Yiseul | - |
dc.contributor.author | Rhyu, Mee-Ra | - |
dc.contributor.author | Fiorucci, Sébastien | - |
dc.contributor.author | Golebiowski, Jerome | - |
dc.date.accessioned | 2021-11-05T08:30:09Z | - |
dc.date.available | 2021-11-05T08:30:09Z | - |
dc.date.created | 2021-11-04 | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 1420-682X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/15760 | - |
dc.description.abstract | Bitter taste receptors (TAS2Rs) are a poorly understood subgroup of G protein-coupled receptors (GPCRs). The experimental structure of these receptors has yet to be determined, and key-residues controlling their function remain mostly unknown. We designed an integrative approach to improve comparative modeling of TAS2Rs. Using current knowledge on class A GPCRs and existing experimental data in the literature as constraints, we pinpointed conserved motifs to entirely re-align the amino-acid sequences of TAS2Rs. We constructed accurate homology models of human TAS2Rs. As a test case, we examined the accuracy of the TAS2R16 model with site-directed mutagenesis and in vitro functional assays. This combination of in silico and in vitro results clarifies sequence-function relationships and proposes functional molecular switches that encode agonist sensing and downstream signaling mechanisms within mammalian TAS2Rs sequences. © 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG. | - |
dc.language | English | - |
dc.publisher | Springer Science and Business Media Deutschland GmbH | - |
dc.title | Functional molecular switches of mammalian G protein-coupled bitter-taste receptors | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00018-021-03968-7 | - |
dc.identifier.scopusid | 2-s2.0-85117734290 | - |
dc.identifier.bibliographicCitation | Cellular and Molecular Life Sciences, v.78, no.23, pp.7605 - 7615 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordAuthor | Bitter taste receptor | - |
dc.subject.keywordAuthor | GPCR | - |
dc.subject.keywordAuthor | Integrative structural biology | - |
dc.subject.keywordAuthor | Structure–function relationships | - |
dc.subject.keywordPlus | BINDING-SITE | - |
dc.subject.keywordPlus | STRUCTURAL BASIS | - |
dc.subject.keywordPlus | GPCR | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | REVEAL | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordPlus | INSIGHTS | - |
dc.subject.keywordPlus | DATABASE | - |
dc.subject.keywordPlus | - | |
dc.citation.endPage | 7615 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 7605 | - |
dc.citation.title | Cellular and Molecular Life Sciences | - |
dc.citation.volume | 78 | - |
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