Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 예경무 | - |
| dc.contributor.author | Beom Yong Lee | - |
| dc.date.accessioned | 2022-03-07T16:00:10Z | - |
| dc.date.available | 2022-03-07T16:00:10Z | - |
| dc.date.issued | 2022 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000593969 | en_US |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/16271 | - |
| dc.description | Cancer cachexia, cisplatin, GDF15, GFRAL, antibody | - |
| dc.description.statementofresponsibility | N | - |
| dc.description.tableofcontents | I. Introduction 1 1.1 Chemotherapy-induced cachexia 1 1.2 Growth differentiation factor 15 (GDF15) 1 1.3 GDF15/GFRAL/RET axis 2 1.4 Aims and objectives 2 II. Materials & Methods 4 2.1 Biopanning 4 2.2 Enzyme-linked immunosorbent assay (ELISA) 4 2.3 Antibody purification 5 2.4 Surface plasmon resonance (SPR) spectrometry 5 2.5 Cells and cell culture 5 2.6 Luciferase reporter assay 6 2.7 Flow cytometry analysis 6 2.8 Immunofluorescence staining 7 2.9 Western blot analysis 7 2.10 Animals and in vivo experiments 8 2.11 Statistical analysis 8 III. Results 9 3.1 GFRAL antagonist antibody was selected by biopanning using human combinatorial antibody phage libraries 9 3.2 Antibody A11 binds to GFRAL and potently suppresses the GDF15/GFRAL/RET axis 12 3.3 Antibody A11 attenuates cisplatin-induced cachexia through inhibition of the GDF15/GFRAL/RET axis in vivo 15 3.4 Antibody A11 alleviates cisplatin-induced cachexia in melanoma-bearing mice 19 IV. Discussion 24 V. References 26 VI. Summary 28 |
- |
| dc.format.extent | 28 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.subject | Cancer cachexia, cisplatin, GDF15, GFRAL, antibody | - |
| dc.title | Development of a GFRAL antagonistic antibody for the amelioration of cancer cachexia | - |
| dc.title.alternative | 암 악액질 증상 개선을 위한 GFRAL 길항 항체 개발 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/thesis.200000593969 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | New Biology | - |
| dc.contributor.coadvisor | Byung-Hoon Lee | - |
| dc.date.awarded | 2022/02 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.NM 이44 202202 | - |
| dc.date.accepted | 1/21/22 | - |
| dc.contributor.alternativeDepartment | 뉴바이올로지전공 | - |
| dc.embargo.liftdate | 20270228 | - |
| dc.contributor.affiliatedAuthor | Beom Yong Lee | - |
| dc.contributor.affiliatedAuthor | Kyungmoo Yea | - |
| dc.contributor.affiliatedAuthor | Byung-Hoon Lee | - |
| dc.contributor.alternativeName | 이범용 | - |
| dc.contributor.alternativeName | Kyungmoo Yea | - |
| dc.contributor.alternativeName | 이병훈 | - |
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