Title

Identification and characterization of a MYB transcription factor, AT3G as a positive regulator of leaf senescence in Arabidopsis

DGIST Authors

Daesang You ; Hye Ryun Woo ; Chang-Hun Lee

Advisor

우혜련

Co-Advisor(s)

Chang-Hun Lee

Issued Date

2022

Awarded Date

2022/02

Citation

Daesang You. (2022). Identification and characterization of a MYB transcription factor, AT3G as a positive regulator of leaf senescence in Arabidopsis. doi: 10.22677/thesis.200000597133

Type

Thesis

Subject

MYB transcription factor, Leaf senescence, Anthocyanin, Arabidopsis

Description

MYB transcription factor, Leaf senescence, Anthocyanin, Arabidopsis

Table Of Contents

Ⅰ. Introduction 1
1.1 Leaf senescence 1
1.2 Anthocyanin accumulation 1
1.3 Relationship between anthocyanin accumulation and leaf senescence 2
Ⅱ. Methods 4
2.1 Plant materials and growth conditions 4
2.2 Leaf senescence assays 4
2.3 Measurement of chlorophyll and anthocyanin content 4
2.4 RNA extraction 4
2.5 Gene expression analysis 4
2.6 Split luciferase assay 5
Ⅲ. Results 6
3.1 Identification of a candidate transcription factor, AT3G 6
3.2 Dark-induced leaf senescence phenotypes of AT3G-KO and AT3G-OX leaves 8
3.3 Phenotypes of AT3G-KO and AT3G-OX under HL stress condition 9
3.4 Protein-protein interaction between AT3G and PAP1 12
Ⅳ. Discussion 13

URI

http://dgist.dcollection.net/common/orgView/200000597133
http://hdl.handle.net/20.500.11750/16288

DOI

10.22677/thesis.200000597133

Degree

Master

Department

New Biology

Publisher

DGIST

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