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Preparation of a sensing membrane for C-reactive protein

Title
Preparation of a sensing membrane for C-reactive protein
Authors
Kim, Hyun-ChulLee, Soo-KeunShon, Young-SooLyu, Hong-KunJeong, Sang Won
DGIST Authors
Kim, Hyun-ChulLee, Soo-Keun; Shon, Young-Soo; Lyu, Hong-KunJeong, Sang Won
Issue Date
2006-10-10
Citation
IUPAC International Symposium on Advanced Polymers for Emerging Technologies, 71-75
Type
Conference
ISSN
1022-1360
Abstract
11,11′-dithiobis(undecanyl succinate) (DS11SACOOH) was synthesized to develop a new surface for immobilization of proteins or antibodies on a gold substrate. DS11SACOOH is a bifunctional linker that has a disulfide group to form SAM (self-assembled monolayer) on a gold substrate and two carboxylic moieties for reaction with biomolecules such as peptide, DNA, etc. After preparation of SAM on a gold-coated quartz cell with DS11SACOOH, the SAM surface was activated with NHS (N-hydroxysuccimide). The terminal NHS ester was reacted with anti-CRP (C-reactive protein). The binding behavior of CRP on the surface was monitored by use of SPR (surface plasmon resonance) sensor system. Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA.
URI
http://hdl.handle.net/20.500.11750/16585
DOI
10.1002/masy.200750312
Publisher
John Wiley & Sons Ltd.
Related Researcher
Files:
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Collection:
Division of Biotechnology2. Conference Papers
Division of Energy Technology2. Conference Papers
ETC2. Conference Papers
Division of Electronics & Information System2. Conference Papers


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