Preparation of a sensing membrane for C-reactive protein
- Title
- Preparation of a sensing membrane for C-reactive protein
- Author(s)
- Kim, Hyun-Chul ; Lee, Soo-Keun ; Shon, Young-Soo ; Lyu, Hong-Kun ; Jeong, Sang Won
- Issued Date
- 2006-10-10
- Citation
- IUPAC International Symposium on Advanced Polymers for Emerging Technologies, pp.71 - 75
- Type
- Conference Paper
- ISSN
- 1022-1360
- Abstract
- 11,11′-dithiobis(undecanyl succinate) (DS11SACOOH) was synthesized to develop a new surface for immobilization of proteins or antibodies on a gold substrate. DS11SACOOH is a bifunctional linker that has a disulfide group to form SAM (self-assembled monolayer) on a gold substrate and two carboxylic moieties for reaction with biomolecules such as peptide, DNA, etc. After preparation of SAM on a gold-coated quartz cell with DS11SACOOH, the SAM surface was activated with NHS (N-hydroxysuccimide). The terminal NHS ester was reacted with anti-CRP (C-reactive protein). The binding behavior of CRP on the surface was monitored by use of SPR (surface plasmon resonance) sensor system. Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA.
- Publisher
- John Wiley & Sons Ltd.
- Related Researcher
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Kim, Hyun-Chul
- Research Interests Nanomedicine; Polymer Drug Conjugates; Drug Delivery System
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