Title

Development of miniaturized Microfluidic Electrochemical Nano-Biosensor platforms for the detection of various Biomarkers.

Alternative Title

다양한 바이오 마커 검출을 위한 미세 유체 전기 화학 나노 바이오 센서 플랫폼 개발

DGIST Authors

KRISHNA CHAITANYA SATISH BABU KASTURI ; CheolGi Kim ; Jong-Sung Yu

Advisor

김철기

Co-Advisor(s)

Jong-Sung Yu

Issued Date

2021

Awarded Date

2021/02

Citation

KRISHNA CHAITANYA SATISH BABU KASTURI. (2021). Development of miniaturized Microfluidic Electrochemical Nano-Biosensor platforms for the detection of various Biomarkers. doi: 10.22677/thesis.200000364302

Type

Thesis

Subject

Microfluidics, Biosensors, Lab-On-a Chip (LOC), electrophoresis, Point-of-Care device (POCD), Pneumatic valves, beta-amyloid, DNA, RNA, biomarker, 미세 유체, 랩 온 어 칩(LOP), 전기 화학 센서, 바이오 마커, 알츠하이머 병(AD), 스퍼터링, 채널, 밸브, 포토 리소그래피, 공압, 나노 물질, 그래핀, 진단, 다중 분석, 동시 감지, 항체, 항원, 고정, 하이브리드

Table Of Contents

1. Introduction 1
1.1. Microfluidics 2
1.2. Electrochemistry 3
1.3. Biosensors 8
1.4. Microfluidic electrochemical biosensing 14
2. Electrochemical biosensor based on naturally reduced rGO/Au nanocomposite for the sensitive detection of the miRNA-122 biomarker 18
2.1. Introduction 18
2.2. Experimental details of the biosensor 20
2.2.1. Materials and chemicals 20
2.2.2. Instruments required for biosensing of miRNA-122 20
2.2.3. Synthesis of rGO/Au nanocomposite 21
2.2.4. Fabrication of rGO/Au modified electrode 22
2.2.5. Probe-DNA immobilization and target miRNA hybridization 22
2.2.6. Electrochemical measurement 23
2.3. Results and discussion 24
2.3.1. Characterization of rGO/Au nanocomposites 24
2.3.2. Time optimization for immobilization and hybridization 28
2.3.3. Immobilization and hybridization studies 29
2.3.4. DPV detection of miRNA-122 biomarker 31
2.3.5. Biomarker selectivity test 32
2.4. Stability and reproducibility of the biosensor 33
2.5. Conclusions 35
3. Experimental techniques and fabrication of microfluidic electrochemical biosensors 37
3.1. Materials required for the fabrication of microfluidic electrochemical biosensors 37
3.1.1. Lithography of Microfluidic compartments 37
3.1.2. Pneumatic microvalve fabrication 38
3.1.3. Microfluidic channel fabrication 38
3.1.4. Electrodes Fabrication 38
3.2. Characterization techniques 40
3.2.1. Scanning Electron Microscope (SEM) 41
3.2.2. Atomic Force microscope (AFM) 42
3.2.3. Autolab 42
3.3. Experimental setup 42
3.4. Lab-on-a-chip-based electrochemical device for the detection beta-amyloid detection 44
4. Lab-on-a-chip based electrochemical device for simultaneous detection of multiple biomarkers 58
4.1. Construction of the miniaturized electrochemical device 59
4.2. Lab-on-a-chip based twelve electrode multiplex analysis for the detection of multiple biomarkers 63
4.3. Surface-enhanced electrode biosensors 66
Conclusion 68
References 70
List of Publications 86
Korean abstract(요약문) 87

URI

http://dgist.dcollection.net/common/orgView/200000364302
http://hdl.handle.net/20.500.11750/16690

DOI

10.22677/thesis.200000364302

Degree

Doctor

Department

Emerging Materials Science

Publisher

DGIST

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