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Real-Time Imaging of Cell Signaling in Response to Spatiotemporal Controlled Gasotransmitter Stimulation

Title
Real-Time Imaging of Cell Signaling in Response to Spatiotemporal Controlled Gasotransmitter Stimulation
Alternative Title
시공간적으로 조절된 기체신호전달물질 자극에 따른 세포신호의 실시간 관찰
Author(s)
Sangwon Shin
DGIST Authors
Sangwon ShinDaeha SeoByung-hoon Lee
Advisor
서대하
Co-Advisor(s)
Byung-hoon Lee
Issued Date
2022
Awarded Date
2022/08
Type
Thesis
Subject
Gasotransmitter (기체신호전달물질), kinase translocation reporter (효소 이동 리포터), ERK signaling (ERK 신호), ROS (활성산소종), RNS (활성질소종)
Description
Gasotransmitter (기체신호전달물질), kinase translocation reporter (효소 이동 리포터), ERK signaling (ERK 신호), ROS (활성산소종), RNS (활성질소종)
Abstract
Table Of Contents
Overview 1
Ⅰ. Imaging of Cellular Damage of ROS from Photocatalysis 5
1. Introduction 5
2. Materials and Methods 6
3. Result and Discussions 11
4. Conclusion 28
Ⅱ. Imaging of Signaling Dynamics in Live cell using Kinase Translocation Reporter 29
1. Introduction 29
2. Materials and Methods 31
3. Result and Discussions 36
4. Conclusion 50
Ⅲ. Real-time imaging of cell signaling in response to different ROS stimulation frequency 51
1. Introduction 51
2. Materials and Methods 53
3. Result and Discussions 55
4. Conclusion 70
Abbreviation. 71
Reference. 72
URI
http://dgist.dcollection.net/common/orgView/200000628895

http://hdl.handle.net/20.500.11750/16786
DOI
10.22677/thesis.200000628895
Degree
Doctor
Department
Department of Physics and Chemistry
Publisher
DGIST
Related Researcher
  • 서대하 Seo, Daeha 화학물리학과
  • Research Interests Synthetic Chemistry of Nanomaterials; Biophysics; Cell biology
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Department of Physics and Chemistry Theses Ph.D.

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