Title

Real-Time Imaging of Cell Signaling in Response to Spatiotemporal Controlled Gasotransmitter Stimulation

Alternative Title

시공간적으로 조절된 기체신호전달물질 자극에 따른 세포신호의 실시간 관찰

DGIST Authors

Sangwon Shin ; Daeha Seo ; Byung-hoon Lee

Advisor

서대하

Co-Advisor(s)

Byung-hoon Lee

Issued Date

2022

Awarded Date

2022/08

Citation

Sangwon Shin. (2022). Real-Time Imaging of Cell Signaling in Response to Spatiotemporal Controlled Gasotransmitter Stimulation. doi: 10.22677/thesis.200000628895

Type

Thesis

Subject

Gasotransmitter (기체신호전달물질), kinase translocation reporter (효소 이동 리포터), ERK signaling (ERK 신호), ROS (활성산소종), RNS (활성질소종)

Description

Gasotransmitter (기체신호전달물질), kinase translocation reporter (효소 이동 리포터), ERK signaling (ERK 신호), ROS (활성산소종), RNS (활성질소종)

Abstract

Table Of Contents

Overview 1
Ⅰ. Imaging of Cellular Damage of ROS from Photocatalysis 5
1. Introduction 5
2. Materials and Methods 6
3. Result and Discussions 11
4. Conclusion 28
Ⅱ. Imaging of Signaling Dynamics in Live cell using Kinase Translocation Reporter 29
1. Introduction 29
2. Materials and Methods 31
3. Result and Discussions 36
4. Conclusion 50
Ⅲ. Real-time imaging of cell signaling in response to different ROS stimulation frequency 51
1. Introduction 51
2. Materials and Methods 53
3. Result and Discussions 55
4. Conclusion 70
Abbreviation. 71
Reference. 72

URI

http://dgist.dcollection.net/common/orgView/200000628895
http://hdl.handle.net/20.500.11750/16786

DOI

10.22677/thesis.200000628895

Degree

Doctor

Department

Department of Physics and Chemistry

Publisher

DGIST

Show Full Item Record