Overview 1 Ⅰ. Imaging of Cellular Damage of ROS from Photocatalysis 5 1. Introduction 5 2. Materials and Methods 6 3. Result and Discussions 11 4. Conclusion 28 Ⅱ. Imaging of Signaling Dynamics in Live cell using Kinase Translocation Reporter 29 1. Introduction 29 2. Materials and Methods 31 3. Result and Discussions 36 4. Conclusion 50 Ⅲ. Real-time imaging of cell signaling in response to different ROS stimulation frequency 51 1. Introduction 51 2. Materials and Methods 53 3. Result and Discussions 55 4. Conclusion 70 Abbreviation. 71 Reference. 72