Ⅰ. Introduction 1 1.1 Cell manipulation for single cell analysis 3 1.2 Spintronic micro-devices for magnetic material manipulation 11 1.3 Spintrophoretic circuit 15 1.4 Mechanical-Barkhausen analysis by discontinuity of bead movement 20 Ⅱ. Experimental methods 24 1. Fabrication of On-chip micromagnet 25 1.1 Lithography 25 1.1.1 Photolithography 27 1.1.2 Thermal scanning probe lithography (t-SPL) 29 1.1.3 Electron beam lithography 31 1.2 Magnetron sputtering 33 2. Domain structures of micromagnets 35 3. Single cell conjugation with magnetic particles 37 4. Experimental set-up 41 5. Data and image analysis methods 41 Ⅲ. Theoretical background 42 1. Magnetic energy in the micromagnet 43 2.Magnetic domain 46 3. Numerical calculations 48 3.1 Magnetization 48 3.2. Driving forces on magnetic particles 49 3.3. Simulation of magnetic particles trajectories 52 4. Mechanical-Barkhausen analysis 54 Ⅳ. Result and discussion 57 1. Analysis of Momentary Mechanical-Barkhausen 57 1.1 Discontinuous movement of magnetic domain on Disk shaped micromagnet 57 1.2 Discontinuous movement of magnetic domain on Ellipse shaped micromagnet 61 2. Control of Periodic Mechanical-Barkhausen 64 2.1 Controlling discontinuous motion of magnetic particles using cracks 64 2.2 Controlling physical properties of magnetically labeled micro-object using Ellipse curvature 76 3. Magnetophoretic application based on Mechanical-Barkhausen analysis 91 3.1 Flower shaped Junction in Spintrophoresis POCT application 91 3.2 Spintrophoretic micro-distributor for controlled clustering of cells 95 Ⅴ. Summary and Future Perspectives 105 1 Summary 106 2. Future Perspectives 108 Reference 110 요약문 115 List of Publications 117
