Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Yoo Seok | ko |
dc.contributor.author | Jung, Hee Kyoung | ko |
dc.contributor.author | Jeon, Won Bae | ko |
dc.contributor.author | Seo, Hwa Jung | ko |
dc.contributor.author | Hong, Joo Heon | ko |
dc.date.available | 2017-07-05T09:01:49Z | - |
dc.date.created | 2017-04-20 | - |
dc.date.issued | 2010-04 | - |
dc.identifier.citation | Journal of the Korean Society of Food Science and Nutrition, v.39, no.4, pp.595 - 601 | - |
dc.identifier.issn | 1226-3311 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/2498 | - |
dc.description.abstract | This study was conducted to detect and identify Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella enterica subsp. using simultaneous multiplex polymerase chain reaction (multiplex PCR) assay. 23S rRNA partial gene (S. aureus), tox R gene (V. parahaemolyticus), and inv A gene (S. enterica subsp.) as diagnostic marker gene were suggested, and their amplicon sizes were 482 bp, 368 bp, and 284 bp, respectively. Non specific amplicons by STA-5F/STA-5R primer, ToxR-F/ToxR-R primer, and 139/141 primer were not observed in genomic DNA of pathogen bacteria as Bacillus cereus, Listeria monocytogenes, Escherichia coli, Proteus vulgaris, Streptococcus pyogenes, Candida albicans, and Shigella sonnei. The extracted crude DNA of targeted bacteria was detected as PCR template successfully. The detection limits were 105~104 CFU/mL and 10 pg of purified genomic DNA of S. aureus, V. parahaemolyticus, and S. enterica subsp. by using simultaneous multiplex PCR. | - |
dc.language | Korean | - |
dc.publisher | 한국식품영양과학회 | - |
dc.subject | Bacillus Cereus | - |
dc.subject | Bacteria (Microorganisms) | - |
dc.subject | Candida Albicans | - |
dc.subject | Escherichia Coli | - |
dc.subject | Listeria Monocytogenes | - |
dc.subject | Multiplex Pcr | - |
dc.subject | Proteus Vulgaris | - |
dc.subject | Salmonella Enterica | - |
dc.subject | Salmonella Enterica Subsp. | - |
dc.subject | Shigella Sonnei | - |
dc.subject | Staphylococcus Aureus | - |
dc.subject | Streptococcus Pyogenes | - |
dc.subject | Vibrio Parahaemolyticus | - |
dc.title | Multiplex Polymerase Chain Reaction(PCR)법을 이용한 Staphylococcus aureus, Salmonella enterica subsp., Vibrio parahaemolyticus의 다중동시검출 | - |
dc.title.alternative | Simultaneous Detection of Staphylococcus aureus, Salmonella enterica subsp., Vibrio parahaemolyticus by Multiplex Polymerase Chain Reaction | - |
dc.type | Article | - |
dc.identifier.doi | 10.3746/jkfn.2010.39.4.595 | - |
dc.identifier.scopusid | 2-s2.0-77957691031 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.kciid | ART001440046 | - |
dc.contributor.nonIdAuthor | Jeong, Yoo Seok | - |
dc.contributor.nonIdAuthor | Jung, Hee Kyoung | - |
dc.contributor.nonIdAuthor | Hong, Joo Heon | - |
dc.identifier.citationVolume | 39 | - |
dc.identifier.citationNumber | 4 | - |
dc.identifier.citationStartPage | 595 | - |
dc.identifier.citationEndPage | 601 | - |
dc.identifier.citationTitle | Journal of the Korean Society of Food Science and Nutrition | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Jeon, Won Bae | - |
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