Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Jones, Alexander M. | - |
dc.contributor.author | Xuan, Yuanhu | - |
dc.contributor.author | Xu, Meng | - |
dc.contributor.author | Wang, Rui-Sheng | - |
dc.contributor.author | Ho, Cheng-Hsun | - |
dc.contributor.author | Lalonde, Sylvie | - |
dc.contributor.author | You, Chang Hun | - |
dc.contributor.author | Sardi, Maria I. | - |
dc.contributor.author | Parsa, Saman A. | - |
dc.contributor.author | Smith-Valle, Erika | - |
dc.contributor.author | Su, Tianying | - |
dc.contributor.author | Frazer, Keith A. | - |
dc.contributor.author | Pilot, Guillaume | - |
dc.contributor.author | Pratelli, Rejane | - |
dc.contributor.author | Grossmann, Guido | - |
dc.contributor.author | Acharya, Biswa R. | - |
dc.contributor.author | Hu, Heng-Cheng | - |
dc.contributor.author | Engineer, Cawas | - |
dc.contributor.author | Villiers, Florent | - |
dc.contributor.author | Ju, Chuanli | - |
dc.contributor.author | Takeda, Kouji | - |
dc.contributor.author | Su, Zhao | - |
dc.contributor.author | Dong, Qunfeng | - |
dc.contributor.author | Assmann, Sarah M. | - |
dc.contributor.author | Chen, Jin | - |
dc.contributor.author | Kwak, June M. | - |
dc.contributor.author | Schroeder, Julian I. | - |
dc.contributor.author | Albert, Reka | - |
dc.contributor.author | Rhee, Seung Y. | - |
dc.contributor.author | Frommer, Wolf B. | - |
dc.date.available | 2017-07-11T05:26:16Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2014-05-16 | - |
dc.identifier.issn | 0036-8075 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/2653 | - |
dc.description.abstract | Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 106 pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, >99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split-green flourescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms. | - |
dc.publisher | American Association for the Advancement of Science | - |
dc.title | Border Control-A Membrane-Linked Interactome of Arabidopsis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1126/science.1251358 | - |
dc.identifier.scopusid | 2-s2.0-84900396278 | - |
dc.identifier.bibliographicCitation | Science, v.344, no.6185, pp.711 - 716 | - |
dc.subject.keywordPlus | ABSCISIC-ACID | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | ENDOCYTOSIS | - |
dc.subject.keywordPlus | NETWORK | - |
dc.subject.keywordPlus | VERSION | - |
dc.subject.keywordPlus | MAP | - |
dc.citation.endPage | 716 | - |
dc.citation.number | 6185 | - |
dc.citation.startPage | 711 | - |
dc.citation.title | Science | - |
dc.citation.volume | 344 | - |
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