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Overexpression of OsCYP19-4 increases tolerance to cold stress and enhances grain yield in rice (Oryza sativa)

Overexpression of OsCYP19-4 increases tolerance to cold stress and enhances grain yield in rice (Oryza sativa)
Yoon, DH[Yoon, Dae Hwa]Lee, SS[Lee, Sang Sook]Park, HJ[Park, Hyun Ji]Lyu, JI[Lyu, Jae Il]Chong, WS[Chong, Won Seog]Liu, JR[Liu, Jang Ryol]Kim, BG[Kim, Beom-Gi]Ahn, JC[Ahn, Jun Cheul]Cho, HS[Cho, Hye Sun]
DGIST Authors
Lyu, JI[Lyu, Jae Il]Liu, JR[Liu, Jang Ryol]
Issued Date
Article Type
ApoplastCold Stress ToleranceCYP19-4ImmunophilinIncreased TilleringOryza Sativa L
AtCYP19-4 (also known as CYP5) was previously identified as interacting in vitro with GNOM, a member of a large family of ARF guanine nucleotide exchange factors that is required for proper polar localization of the auxin efflux carrier PIN1. The present study demonstrated that OsCYP19-4, a gene encoding a putative homologue of AtCYP19-4, was up-regulated by several stresses and showed over 10-fold up-regulation in response to cold. The study further demonstrated that the promoter of OsCYP19-4 was activated in response to cold stress. An OsCYP19-4-GFP fusion protein was targeted to the outside of the plasma membrane via the endoplasmic reticulum as determined using brefeldin A, a vesicle trafficking inhibitor. An in vitro assay with a synthetic substrate oligomer confirmed that OsCYP19-4 had peptidyl-prolyl cis-trans isomerase activity, as was previously reported for AtCYP19-4. Rice plants overexpressing OsCYP19-4 showed cold-resistance phenotypes with significantly increased tiller and spike numbers, and consequently enhanced grain weight, compared with wild-type plants. Based on these results, the authors suggest that OsCYP19-4 is required for developmental acclimation to environmental stresses, especially cold. Furthermore, the results point to the potential of manipulating OsCYP19-4 expression to enhance cold tolerance or to increase biomass. © 2015 The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Oxford University Press
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