Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Shin, Min Sun | - |
dc.contributor.author | You, Sungyong | - |
dc.contributor.author | Kang, Youna | - |
dc.contributor.author | Lee, Naeun | - |
dc.contributor.author | Yoo, Seung-Ah | - |
dc.contributor.author | Park, Kieyoung | - |
dc.contributor.author | Kang, Ki Soo | - |
dc.contributor.author | Kim, Sang Hyun | - |
dc.contributor.author | Mohanty, Subhasis | - |
dc.contributor.author | Shaw, Albert C. | - |
dc.contributor.author | Montgomery, Ruth R. | - |
dc.contributor.author | Hwang, Daehee | - |
dc.contributor.author | Kang, Insoo | - |
dc.date.available | 2017-07-11T05:46:01Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2015-09-15 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/2846 | - |
dc.description.abstract | DNA methylation is an epigenetic mechanism that modulates gene expression in mammalian cells including T cells. Memory T cells are heterogeneous populations. Human effector memory (EM) CD8+ T cells in peripheral blood contain two cell subsets with distinct traits that express low and high levels of the IL-7Rα. However, epigenetic mechanisms involved in defining such cellular traits are largely unknown. In this study, we use genome-wide DNA methylation and individual gene expression to show the possible role of DNA methylation in conferring distinct traits of chemotaxis and inflammatory responses in human IL-7Rαlow and IL-7Rαhigh EM CD8+ T cells. In particular, IL-7Rαlow EMCD8+ T cells had increased expression of CX3CR1 along with decreased DNA methylation in the CX3CR1 gene promoter compared with IL-7Rαhigh EM CD8+ T cells. Altering the DNA methylation status of the CX3CR1 gene promoter changed its activity and gene expression. IL-7Rαlow EM CD8+ T cells had an increased migratory capacity to the CX3CR1 ligand fractalkine compared with IL-7Rαhigh EM CD8+ T cells, suggesting an important biological outcome of the differential expression of CX3CR1. Moreover, IL-7Rαlow EM CD8+ T cells induced fractalkine expression on endothelial cells by producing IFN-γ and TNF-α, forming an autocrine amplification loop. Overall, our study shows the role of DNA methylation in generating unique cellular traits in human IL-7Rαlow and IL-7Rαhigh EM CD8+ T cells, including differential expression of CX3CR1, as well as potential biological implications of this differential expression. © 2015 by The American Association of Immunologists, Inc. | - |
dc.publisher | American Association of Immunologists | - |
dc.title | DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7R alpha(low) and IL-7R alpha(high) Effector Memory CD8(+) T Cells with Distinct Migratory Capacities to the Fractalkine | - |
dc.type | Article | - |
dc.identifier.doi | 10.4049/jimmunol.1500877 | - |
dc.identifier.scopusid | 2-s2.0-84941768077 | - |
dc.identifier.bibliographicCitation | Journal of Immunology, v.195, no.6, pp.2861 - 2869 | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | ASTHMA | - |
dc.subject.keywordPlus | ATHEROSCLEROSIS | - |
dc.subject.keywordPlus | Biosynthesis | - |
dc.subject.keywordPlus | CD8-Positive T-Lymphocytes | - |
dc.subject.keywordPlus | CD8+ T Lymphocyte | - |
dc.subject.keywordPlus | Cell Adhesion | - |
dc.subject.keywordPlus | Cell Assay | - |
dc.subject.keywordPlus | Cell Culture | - |
dc.subject.keywordPlus | Cells, Cultured | - |
dc.subject.keywordPlus | CHemOKINE-RECEPTOR | - |
dc.subject.keywordPlus | Chemokine Cx3Cl1 | - |
dc.subject.keywordPlus | CHemOKINE RECEPTOR | - |
dc.subject.keywordPlus | Chemokine Receptor CX3CR1 | - |
dc.subject.keywordPlus | Chemotaxis | - |
dc.subject.keywordPlus | Chromatin | - |
dc.subject.keywordPlus | Controlled Study | - |
dc.subject.keywordPlus | CX3CR1 Protein, Human | - |
dc.subject.keywordPlus | DNA Methylation | - |
dc.subject.keywordPlus | Effector Cell | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | Endothelium Cell | - |
dc.subject.keywordPlus | Epigenetics | - |
dc.subject.keywordPlus | Fractalkine | - |
dc.subject.keywordPlus | Gamma Interferon | - |
dc.subject.keywordPlus | Gene Expression | - |
dc.subject.keywordPlus | Genetic Association | - |
dc.subject.keywordPlus | Genetics | - |
dc.subject.keywordPlus | Human | - |
dc.subject.keywordPlus | Human Cell | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | IFN-GAMMA GENE | - |
dc.subject.keywordPlus | Immune Response | - |
dc.subject.keywordPlus | Immunologic Memory | - |
dc.subject.keywordPlus | Immunological Memory | - |
dc.subject.keywordPlus | Immunology | - |
dc.subject.keywordPlus | Interferon-Gamma | - |
dc.subject.keywordPlus | INTERLEUKIN-7 | - |
dc.subject.keywordPlus | Interleukin-7 Receptor, Alpha Chain | - |
dc.subject.keywordPlus | Interleukin 7 Receptor | - |
dc.subject.keywordPlus | Interleukin 7 Receptor Alpha | - |
dc.subject.keywordPlus | LUPUS NEPHRITIS | - |
dc.subject.keywordPlus | Lymphocyte Migration | - |
dc.subject.keywordPlus | Memory Cell | - |
dc.subject.keywordPlus | Metabolism | - |
dc.subject.keywordPlus | Molecular Dynamics | - |
dc.subject.keywordPlus | Priority Journal | - |
dc.subject.keywordPlus | Promoter Region | - |
dc.subject.keywordPlus | Promoter Regions, Genetic | - |
dc.subject.keywordPlus | Protein Determination | - |
dc.subject.keywordPlus | Protein Expression | - |
dc.subject.keywordPlus | Protein Function | - |
dc.subject.keywordPlus | Receptors, Chemokine | - |
dc.subject.keywordPlus | Receptors, Interleukin-7 | - |
dc.subject.keywordPlus | T-Lymphocyte Subsets | - |
dc.subject.keywordPlus | T Lymphocyte Subpopulation | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | Tumor Necrosis Factor-Alpha | - |
dc.subject.keywordPlus | Tumor Necrosis Factor Alpha | - |
dc.citation.endPage | 2869 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 2861 | - |
dc.citation.title | Journal of Immunology | - |
dc.citation.volume | 195 | - |
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