Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy)32+-Doped Silica Nanoparticles and Calix[4]crown-5 Self-Assembled Monolayers
- Title
- Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy)32+-Doped Silica Nanoparticles and Calix[4]crown-5 Self-Assembled Monolayers
- Author(s)
- Kim, Yang-Rae ; Seo, Hyo-Ju ; Oh, Jeong-Wook ; Lim, Hyunchang ; Kim, Tae Hyun ; Kim, Hasuck
- Issued Date
- 2013-04
- Citation
- Electroanalysis, v.25, no.4, pp.1056 - 1063
- Type
- Article
- Author Keywords
- Electrogenerated chemiluminescence ; Immunoassay ; Immunoglobulin G ; Silica nanoparticles ; Self-assembled monolayers ; Quartz crystal microbalance
- Keywords
- PROTEIN ; ELECTROCHEMILUMINESCENCE ; BENZO-15-CROWN-5 ; FILMS ; DNA
- ISSN
- 1040-0397
- Abstract
- An electrogenerated chemiluminescence (ECL)-based immunosensor for the detection of immunoglobulin G (IgG) has been fabricated using Ru(bpy)3 2+-doped silica nanoparticles and calix[4]crown-5 self-assembled monolayers (SAMs). Ru(bpy)3 2+-doped silica nanoparticles are prepared by the water-in-oil (W/O) microemulsion method. ProLinker B, a commercially available thiolated calix[4]crown-5 derivative, is utilized for the immobilization of anti-immunoglobulin G (Anti-IgG) on a gold electrode. The concentration of IgG is measured using a sandwich-type ECL immunosensor based on the proposed immunosensor. The ECL intensity is linearly proportional to the IgG concentration over the concentration range 5-30μgmL-1. The detection limit of IgG is 1.5μgmL-1. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Publisher
- Wiley-VCH Verlag
- Files in This Item:
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- Appears in Collections:
- ETC 1. Journal Articles
- Department of Energy Science and Engineering ETC 1. Journal Articles
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