Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Han, Sang Hee | - |
dc.contributor.author | Kim, Sang Kyu | - |
dc.contributor.author | Park, Kyoungsook | - |
dc.contributor.author | Yi, So Yeon | - |
dc.contributor.author | Park, Hye-Jung | - |
dc.contributor.author | Lyu, Hong-Kun | - |
dc.contributor.author | Kim, Moonil | - |
dc.contributor.author | Chung, Bong Hyun | - |
dc.date.available | 2017-07-11T07:12:23Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2010-04-14 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/3527 | - |
dc.description.abstract | We assessed the abilities of wild p53 and mutant p53 proteins to interact with the consensus DNA-binding sequence using a MOSFET biosensor. This is the first report in which mutant p53 has been detected on the basis of DNA-protein interaction using a FET-type biosensor. In an effort to evaluate the performance of this protocol, we constructed the core domain of wild p53 and mutant p53 (R248W), which is DNA-binding-defective. After the immobilization of the cognate DNA to the sensing layer, wild p53 and mutant p53 were applied to the DNA-coated gate surface, and subsequently analyzed using a semiconductor analyzer. As a consequence, a significant up-shift in drain current was noted in response to wild p53, but not mutant p53, thereby indicating that sequence-specific DNA-protein interactions could be successfully monitored using a field-effect-based biosensor. These data also corresponded to the results obtained using surface plasmon resonance (SPR) measurements. Taken together, our results show that a FET-type biosensor might be promising for the monitoring of mutant p53 on the basis of its DNA-binding activity, providing us with very valuable insights into the monitoring for diseases, particularly those associated with DNA-protein binding events. © 2010 Elsevier B.V. | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Detection of mutant p53 using field-effect transistor biosensor | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.aca.2010.03.006 | - |
dc.identifier.wosid | 000277329300012 | - |
dc.identifier.scopusid | 2-s2.0-77951209238 | - |
dc.identifier.bibliographicCitation | Analytica Chimica Acta, v.665, no.1, pp.79 - 83 | - |
dc.subject.keywordAuthor | Metal oxide semiconductor field-effect transistor | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | p53 | - |
dc.subject.keywordAuthor | Mutant p53 | - |
dc.subject.keywordAuthor | DNA-binding domain | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | Binding | - |
dc.subject.keywordPlus | Biochemistry | - |
dc.subject.keywordPlus | Biosensing Techniques | - |
dc.subject.keywordPlus | Biosensor | - |
dc.subject.keywordPlus | BIOSENSORS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | CHARGE | - |
dc.subject.keywordPlus | Controlled Study | - |
dc.subject.keywordPlus | DATABASE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | DNA-Binding | - |
dc.subject.keywordPlus | DNA-Binding Domain | - |
dc.subject.keywordPlus | DNA-Protein Interaction | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | Drain Current | - |
dc.subject.keywordPlus | Fet-Type Biosensors | - |
dc.subject.keywordPlus | Field-Effect | - |
dc.subject.keywordPlus | Field Effect Transistor | - |
dc.subject.keywordPlus | Field Effect Transistors | - |
dc.subject.keywordPlus | Gene | - |
dc.subject.keywordPlus | Gene Mutation | - |
dc.subject.keywordPlus | Genes | - |
dc.subject.keywordPlus | HUMAN TUMORS | - |
dc.subject.keywordPlus | Mesfet Devices | - |
dc.subject.keywordPlus | Metal Oxide Semiconductor Field-Effect Transistor | - |
dc.subject.keywordPlus | Metal Oxide Semiconductor Field-Effect Transistors | - |
dc.subject.keywordPlus | Metallic Compounds | - |
dc.subject.keywordPlus | Monitoring | - |
dc.subject.keywordPlus | MOS-FET | - |
dc.subject.keywordPlus | Mos Devices | - |
dc.subject.keywordPlus | Mutagenesis, Site-Directed | - |
dc.subject.keywordPlus | Mutant P53 | - |
dc.subject.keywordPlus | Mutant Protein | - |
dc.subject.keywordPlus | Mutation | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | P53 Protein | - |
dc.subject.keywordPlus | Priority Journal | - |
dc.subject.keywordPlus | Protein Binding | - |
dc.subject.keywordPlus | Protein DNA Interaction | - |
dc.subject.keywordPlus | Protein Immobilization | - |
dc.subject.keywordPlus | Protein P53 | - |
dc.subject.keywordPlus | Protein Structure, Tertiary | - |
dc.subject.keywordPlus | Proteins | - |
dc.subject.keywordPlus | Recombinant Proteins | - |
dc.subject.keywordPlus | Semiconductor Analyzer | - |
dc.subject.keywordPlus | Sensing Layers | - |
dc.subject.keywordPlus | Surface Plasmon Resonance | - |
dc.subject.keywordPlus | Surface Property | - |
dc.subject.keywordPlus | Transistors, Electronic | - |
dc.subject.keywordPlus | Tumor Suppressor Protein P53 | - |
dc.citation.endPage | 83 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 79 | - |
dc.citation.title | Analytica Chimica Acta | - |
dc.citation.volume | 665 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.type.docType | Article | - |
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